Description
TheantibodyhasaprovenstrongBiotin-Streptavidin/HRPimmunostainingata1/500-1/1000dilutioninhumanpancreaticislets.Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodyinanexcessofglucagon.
Preadsorptionofthedilutedantibodywithanexcessofthefollowingsubstanceshadnoeffectonglucagonlabeling:secretin,vasoactiveintestinalpeptide,peptidehistidineisoleucine-27,gastricinhibitorypolypeptide,ratandhumangrowthhormonereleasinghormoneandsomatostatin.
PhotoDescription:IHCimageofalphacellsintheisletsofLangerhansoftheratpancreasstainingforglucagon(above)andcounterstainedwithhemotoxylin(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforfrozensectioning.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:1000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:Chick,Human,Mouse,Pig,Rat,SeaLamprey,Snail,Three-SpinedStickleback(Fish),Turtle
AvailABIlity:InStock
AlternateNames: anti-glucagon
GeneSymbol/ID,Accession#: GCG,2641,280802,397595
RRID: AB_572241