Description
TheantibodyproducesstronglabelingofGHRHatadilutionof1/2,000-1/4,000usingbiotin/streptavidinHRPinrathypothalamus(medianeminence).Optimaldilutionwillvarydependinguponfixation,labelingtehcniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.
CrossreactivityofGHRFantiserumwasexaminedusingthepaperspottechniqueofLarsson(1981).Using2µL,100pmoleamounts,thefollowingsubstancesdidnotreactwithratGHRFantiseradiluted1/500usingthePAPlabelingmethod:glucagon,gastricinhibitorypeptide,secretin,vasoactiveintestinalpeptide,peptidehistidineisoleucine,pancreaticpolypeptide(humanorrat),humanGHRF,somatostatin,insulin,ACTH,motilin,cholecystokininoctapeptide,substanceP,molluscancardioexcitatiorypeptide,gastrin34,andSEROtonin.GHRFantiserumhadaverygoodreactivityusingratGHRFat2µL,100pmoleamounts.
PhotoDescription:IHCimageofneuronsstainingforDBHintheratbrainstem.Thetissuewasfixedwith4%formaldehyde/0.05%glutaraldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:4000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State: Lyophilized
ReactsWith:Rat
AlternateNames: GRF;GHRH;Somatoliberin;Growthhormone-releasinghormone;Sermorelin;Somatocrinin;Somatorelin;Ghrf;growthhormonereleasinghormone,anti-GHRF
GeneSymbol/ID,Accession#: Ghrh,29446
RRID: AB_572239