Description
TheImmunoStargammaaminobutyricacidantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratthalamusandcerebellumusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionsare1/15,000-1/20,000inPBS/0.3%TritonX-100-biotin/avidin-HRPtechnique.
ThespecificityoftheantiserumforGABAwasevaluatedusingacompetitiveinhibitionELISA.WhileconjugatesofGABAcompletelyeliminatelabeling,a1000foldexcessofthefollowingconjugatescouldnotinhibittheantiserumsABIlitytobindGABAconjugate:glutamate,aspartate,betaalanine,tyrosine,taurine,glycineandalanine.
PhotoDescription:IHCimageofneuronsstainingforGABAintheratthalamus(above)andtheratcerebellum(belowleft,lowmagnification;belowright,highmagnification).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:20000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogenandhemotoxylincounterstainintheratthalamus.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:Alligator,ArmADIllo,Bat,BathynomusDoederleini(Crustacean/Isopod),Bird,CaenorhaBDitisElegansNematode(Roundworm),Cat,Drosophila(Fly),DrosophilaMelanogaster(Fly),Ferret,Fish,Frog,Frog(XenopusLaevis),Frog(XenopusTectum),Gerbil,GuineaPig,Hamster,Human,Kitten(Cat),Monkey,Moth,Mouse,Parakeet,Pigeon,PleurobranchaeaJaponica(SeaSlug),Rabbit,Racoon,Rat,Slug,Snail,Turtle,ZebraFinch(Bird)
Availability:InStock
AlternateNames: anti-GABA
RRID: AB_572234
Immunogen:GABA
GeneSymbol:Rdl
EntrezGeneID:39054
NCBIGeneAliases:CG10537,DmRDL,DmRdl,DmelCG10537,GABA,GABA[[A]],"GABA[[A]]receptor"GABAr,LCCH1,RDL,Rd1,gaba,rd