Xanthine/HypoxanthineColorimetric/FluorometricAssayKit.

SensitiveAssay,HTS.

 

KitSummary:

•Detectionmethod-Absorbance(570nm)orFluoroescence(Ex/Em=535/587nm)

•Application-ThekitcandetectXanthine/Hypoxanthinelevelsaslowas0.4µMinvariousBIOLOGicalsamples.

 

Sampletype:

•BodyFluids:serum,plasma,urineetc.

•Animaltissues:liver,muscle,heartetc.

•Cellculture:adherentorsUSPensioncells.

•Cellandtissueculturesupernatant.

 

Features&Benefits:

•Simpleprocedure;takes~40minutes

•Rapid,sensitiveandhigh-throughputadaptable

•KitcontainsallnecessaryreagentsforaccuratemeasurementofXanthine/Hypoxanthinelevels.

 

KitComponents:

•XanthineAssayBuffer

•OxiRedTMProbe(inDMSO)

•XanthineEnzymeMix(Lyophilized)

•Developer(Lyophilized)

•XanthineStandard(Lyophilized)

 

Description:

Xanthine,acatabolicproductofpurinemetabolism,ispresentinbodyfluids,muscletissueandcertainplants.Structurallylikecaffeine,Xanthinehasastimulanteffectandisusedclinicallytotreatthecongestivediseasessuchasasthmaandchronicobstructivepulmonarydisease.XanthineismetabolizedintouricacidandsuperoxidebyXanthineoxidase.Xanthineoxidasedeficiencycausestheraregeneticdisorder-Xanthinuria,andleadstoXanthineaccumulationinurineandblood,whichultimatelyprogressestorenalfailure.RecentstudiesshowthatXanthinelevelsareelevatedfollowingischemicinjury,thusXanthinecanserveasausefulMarkerfortissuehypoxia.EarlydetectionofXanthinealterationinbiologicalfluidsiscrucialformetabolicstudiesandfordiagnosticandtherapeuticmonitoring.InHansABIoMed"sXanthine/HypoxanthineAssaykit,Xanthine/HypoxanthineisspecificallyoxidizedbytheXanthineEnzymeMixtoformanintermediate,whichreactswithDeveloper&Probetoformaproductthatcanbemeasuredcolorimetrically(λ=570nm)orfluorometrically(Ex/Em=535/587nm).Xanthine/HypoxanthineAssaykitisrapid,simpleandsensitive.Thishigh-throughputsuitableassaykitcandetectXanthinelevelsaslowas0.4µMinvariousbiologicalsamples.

 

StorageConditions:-20°C.

 

ShippingConditions:gelpack.

 

USAGE:ForResearchUseOnly!NotForUseinHumans.

HansaBioMed抗体抗体是基础研究，开发人类疾病的诊断测试和疗法的重要工具。包括外泌体的细胞外囊泡（EV）表达的抗原具有3D构象和/或翻译后修饰，通常不同于细胞对应物。因此，目前市场上可买到的大多数抗体都无法以足够的敏感性和特异性识别与EV相关的抗原。HBM与Exosomics Siena Spa合作，已验证了针对常见（CD63，CD81，ALIX）和疾病特异性（癌症和神经退行性疾病）EV标记的多克隆和单克隆抗体清单单克隆AB（9）多克隆AB（5）