ProteasomeActivityAssayKit-100reactions

 

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em350/440nm).

•Sampletype-Wholecelllysates,nuclearandcytoplasmiclysates.

•Speciesreactivity-Mammalian.

•Applications-Specificallymeasuresproteasomeactivityfromotherproteaseactivitypresentinsamples.

 

Features&Benefits:

•Simpleone-stepprocedure;takesonly30-60minutes.

•Accurateandeasytouse.

 

Kitcomponents:

•ProteasomeAssayBuffer.

•ProteasomeSubstrate(InDMSO).

•ProteasomeInhibitor(InDMSO).

•AMCStandard(1mMinDMSO).

•PositiveControl.

 

Description:

Proteasomesareverylarge(20S,26S)proteinassembliesfoundinboththenucleusandcytoplasmofalleucaryotes(andinsomeprocaryotes).TheyareresponsIBLeforthedegradationandrecyclingofproteinswhichhavebeenpreviouslytaggedwithubiquitin.Suchtaggedproteinsaredegradedintopeptidesapproximately7-8aminoacidslongwhicharesubsequentlyfurtherdegraded.The20Sassemblyisthefunctionalproteasestructurewithchymotrypsin-like,trypsin-likeandcaspase-likeproteaseactivities.ProteasomeActivityAssaytakesadvantageofthechymotrypsin-likeactivity,utilizinganAMC-taggedpeptidesubstratewhichreleasesfree,highlyfluorescentAMCinthepresenceofproteolyticactivity.Thekitalsoincludesapositivecontrol(JurkatCelllysatewithsignificantproteasomeactivity)andaspecificproteasomeinhibitorMG-132whichsuppressesallproteolyticactivityduetoproteasomes.Thispermitsdifferentiationofproteasomeactivityfromotherproteaseactivitywhichmaybepresentinsamples.

 

StorageConditions:-20°C.

 

ShippingConditions:gelpack.

 

USAGE:ForResearchUseOnly!NotForUseinHumans.

HansaBioMed抗体抗体是基础研究，开发人类疾病的诊断测试和疗法的重要工具。包括外泌体的细胞外囊泡（EV）表达的抗原具有3D构象和/或翻译后修饰，通常不同于细胞对应物。因此，目前市场上可买到的大多数抗体都无法以足够的敏感性和特异性识别与EV相关的抗原。HBM与Exosomics Siena Spa合作，已验证了针对常见（CD63，CD81，ALIX）和疾病特异性（癌症和神经退行性疾病）EV标记的多克隆和单克隆抗体清单单克隆AB（9）多克隆AB（5）