BrdUCellProliferationAssayKit-200reactions

 

KitSummary:

•Detectionmethod-Absorbance(450nm).

•Applications-Detectionandquantificationofcellproliferationinducedbygrowthfactors,cytokines,mitogens,andnutrients.

•Analysisofcytotoxicandcytostaticcompoundssuchasanticancerdrugs,toxicagentsandotherpharmaceuticals.

•Determinationoftheinhibitoryorstimulatoryeffectsofvariouscompoundsoncellproliferation.

 

SampleType:

•AdherentandsUSPensioncells.

 

Features&Benefits:

•Convenient&non-rADIoactive.

•High-throughputsuitable.

•Highly-sensitive:detectaslowas50-100proliferatingcells.

•Suitableforcellproliferation,chemotaxisandcytotoxicity.

 

KitComponents:

•BrdU(1000X),

•Fixing/DenaturingSolution.

•BrdUDetectionAntibody(300X).

•Anti-mouseHRP-linkedAntibody(2000X).

•AntibodyDiluent.

•WashBuffer(10X).

•TMBSubstrate.

•StopSolution.

 

Description:

5-bromo-2-deoxyuridine(BrdU)isapyrimidineanalog.ItgetsincorporatedintothenewlysynthesizedDNAofproliferatingcellsinplaceofthymidine.BrdUCellProliferationAssayKitdetectsincorporatedBrdUusingamouseanti-BrdUantibody.Ananti-mouseHRP-linkedsecondaryantibodyisusedtodetecttheanti-BrdUantibodyboundtoBrdU,whichisfollowedbyadditionofTMB(aHRPsubstrate).TheextentofcolordevelopmentisproportionaltothequantityofBrdUincorporatedintothecellsandcanbeuseddirectlyasanindicatorofcellproliferation.Comparedtoothercellproliferationassays,thiskitdetectsonlytheproliferatingcellsandnottheseededcells.Thishighlysensitive,non-radioactivekitdetectsaslessas50-100proliferatingcells.

 

StorageConditions:-20°C.

 

ShippingConditions:gelpack.

 

USAGE:ForResearchUseOnly!NotForUseinHumans.

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