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蚂蚁淘在线 / 品牌中心 / HansaBioMed / HansaBioMed/ExoTEST Ready to Use Kit for Overall Exosome capture and quantification from cell culture supernatant (trans

HansaBioMed/ExoTEST Ready to Use Kit for Overall Exosome capture and quantification from cell culture supernatant (trans

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￥7186.40

货号：HBM-RTK-POC/TC

浏览量：127

品牌：HansaBioMed

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商品描述

ExoTESTisasandwichenzyme-linkedimmunoassayforin-vitroexosomequantificationincellculturesupernatant.

Application:

ExosomecaptureandquantificationusingtheExoTESTensuresdose-dependentexosomedetectioninavarietyofsamples.Thequantificationcanbebothrelativeandabsoluteastheassaycalibrationenablesthecalculationoftheapproximateexosomequantityforeachsample.

Comprehensiveexosomeprofilingbyimplementingdifferentcombinationsofcaptureanddetectionantibodies.ExoTESTcanbecustomizedforassessingmultipleantigensinatotalexosomepopulationwithinasample.Informativeproteinprofilesforthesamplesofinterestcanbecombinedwithsubsequentnucleicacid(mRNAormiRNA)extractionanddownstreamanalysisfromcapturedexosomes.

SpecificdiagnosticapplicationsusingtheExoTESTallowsthequantitativeassessmentoftotalcirculatingexosomesandspecificexosomalsub-populations,definedbydifferentiallyexpressedantigensundernormalandpathologicalconditions.

Relatedproducts :

Exosomestandards

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Immunocaptureandisolationtools

HansaBioMed抗体抗体是基础研究，开发人类疾病的诊断测试和疗法的重要工具。包括外泌体的细胞外囊泡（EV）表达的抗原具有3D构象和/或翻译后修饰，通常不同于细胞对应物。因此，目前市场上可买到的大多数抗体都无法以足够的敏感性和特异性识别与EV相关的抗原。HBM与Exosomics Siena Spa合作，已验证了针对常见（CD63，CD81，ALIX）和疾病特异性（癌症和神经退行性疾病）EV标记的多克隆和单克隆抗体清单单克隆AB（9）多克隆AB（5）

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Sampletype	Cellculturesupernatant.
Startingmaterial	Upto100ulofBIOLOGicalsamplecanbeloadedperwell. Concentratedcellculturesupernatantsamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol.
Assaytype	Sandwichenzyme-linkedimmunoassay.
Sensitivity	Thedetectionlimitofanassayislowerthan0.35ugofoverallexosomewhichisanequivalentoflessthan50pgoftargetedexosomesprotein.
Productoverview	ExoTESTReadytoUseKitistheidealplatformforcapturingandthequantifyingexosomesfromcellculturesupernatant. ExoTESTReadytoUseKitfeaturespre-coatedELISAplatewithprimaryantibodiesdetectiondirectedagainstexosome-specificMarkers,namelyα-CD9.Secondaryantibodiesandsubstratereagentsareincludedinthekit. Exosomestandardsforassaycalibrationarealsoincludedinthekit.ExosomestandardsarepurifiedbyultracentrifugationandMICROFILtration,quantifiedforoverallproteincontentandexosomenumberandthenlyophilizedforlong-termstorage. ExoTESTreadytousekitsarereliable,easytouse,worksonavarietyofcommerciallyavailabledetectiontechnologies.
Primaryantibody	α-CD9mouseantibody.
Exosomestandard	LyophilizedexosomesfromCOLO-1Cellculturesupernatant (seedetails).
Assayprotocol	Kitcontainsallthereagentsnecessaryforexosomecaptureandproteintargeteddetection. Assaycalibration:Kitcontainsexosomestandardspreparedfrompurifiedfromproprietaryhumancelllineforassaycalibration.Allexosomestandardsarelyophilizedandquantifiedforoverallproteincontentandexosomesnumber. HansABIoMedalsoprovidesarangeofexosomestandardspurifiedfromdifferenttypesofhumancellsthatcanbepurchasedseparatelyorincludedincustomkitsinordertomeettheneedfordetectionofspecificexosomemarkers.
Shipmentandstorage	Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductinsert.
Expirationdate	12months.