- 外显子-DNA-PS：从血浆和血清中分离循环和EV相关DNA
- 生物化学
- Exo准备
- 细胞增殖和分离分析
- 外显子DNAc PS：从血浆和血清中分离循环和EV相关DNA
- 尿液中冻干纯化外体
- 外显子DNAc-UC：尿液及细胞培养液中循环及EV相关DNA的分离
- 外显子-DNA-UC:尿液和细胞培养液中循环和EV相关DNA的分离
- 单克隆抗体
- 外显子-DNA-UC:尿液和细胞培养液中循环和EV相关DNA的分离
- 外显子DNAc-UC：尿液及细胞培养液中循环及EV相关DNA的分离
- Exo试验：即用ELISA定量试剂盒
- RNA碱性试剂盒
- 冻干纯化血浆外体
- 氧化应激
- 冻干纯化血清外体
- 唾液外体的冻干纯化
- ELISA免疫平板
- 不同细胞系外体的冻干纯化
- 纯EVs：大小排阻色谱柱
- 肿瘤总RNA：肿瘤外体免疫捕获和RNA提取试剂盒
ExoTESTisa sandwichenzyme-linkedimmunoassayforin-vitroexosomequantificationinhumanurine.
Sampletype | Urine |
Startingmaterial | Upto100ulofBIOLOGicalsamplecanbeloadedperwell. Unfractionatedplasmasamplecanbedirectlyusedforcapture. Concentratedurinesamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol. |
Assaytype | Sandwichenzyme-linkedimmunoassay. |
Sensitivity | Thedetectionlimitofanassayislowerthan0.35ugofoverallexosomewhichisanequivalentoflessthan50pgoftargetedexosomesprotein. |
Productoverview | ExoTESTReadytoUseKitistheidealplatformforcapturingandthequantifyingexosomesfrombiologicalsamplessuchasplasmaandurine. ExoTESTReadytoUseKitfeaturespre-coatedELISAplatewithprimaryantibodiesdetectiondirectedagainstexosome-specificMarkers,namelyα-CD9.Secondaryantibodiesandsubstratereagentsareincludedinthekit. Exosomestandardsforassaycalibrationarealsoincludedinthekit.ExosomestandardsarepurifiedbyultracentrifugationandMICROFILtration,quantifiedforoverallproteincontentandexosomenumberandthenlyophilizedforlong-termstorage. ExoTESTreadytousekitsarereliable,easytouse,worksonavarietyofcommerciallyavailabledetectiontechnologies. |
Primaryantibody | α-CD9mouseantibody |
Exosomestandard | Lyophilizedexosomesfromurineofhealthydonors(see details). |
Assayprotocol | Kitcontainsallthereagentsnecessaryforexosomecaptureandproteintargeteddetection. Assaycalibration:Kitcontainsexosomestandardsforassaycalibration.Dependedonthesampletypeused,kitmayincludehumanplasma,serumorurineexosomes.Allexosomestandardsarelyophilizedandquantifiedforoverallproteincontentandexosomesnumber. Thisstandardsenablebothabsoluteandrelativequantificationexosomesinanalyzedsamples. |
Shipmentandstorage | Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductinsert. |
Expirationdate | 12months. |
Application:
ExosomecaptureandquantificationusingtheExoTESTensuresdose-dependentexosomedetectioninavarietyofsamples.Thequantificationcanbebothrelativeandabsoluteastheassaycalibrationenablesthecalculationoftheapproximateexosomequantityforeachsample.
Comprehensiveexosomeprofilingbyimplementingdifferentcombinationsofcaptureanddetectionantibodies.ExoTESTcanbecustomizedforassessingmultipleantigensinatotalexosomepopulationwithinasample.Informativeproteinprofilesforthesamplesofinterestcanbecombinedwithsubsequentnucleicacid(mRNAormiRNA)extractionanddownstreamanalysisfromcapturedexosomes.
SpecificdiagnosticapplicationsusingtheExoTESTallowsthequantitativeassessmentoftotalcirculatingexosomesandspecificexosomalsub-populations,definedbydifferentiallyexpressedantigensundernormalandpathologicalconditions.
Relatedproducts :
Exosomestandards
RNAextractionkit
Immunocaptureandisolationtools
HansaBioMed抗体抗体是基础研究，开发人类疾病的诊断测试和疗法的重要工具。包括外泌体的细胞外囊泡（EV）表达的抗原具有3D构象和/或翻译后修饰，通常不同于细胞对应物。因此，目前市场上可买到的大多数抗体都无法以足够的敏感性和特异性识别与EV相关的抗原。HBM与Exosomics Siena Spa合作，已验证了针对常见（CD63，CD81，ALIX）和疾病特异性（癌症和神经退行性疾病）EV标记的多克隆和单克隆抗体清单单克隆AB（9）多克隆AB（5）
