ProductHighlights:
- Removescytoplasmic,mitochondrial,andchloroplastribosomalRNA(rRNA)inasinglepass
- SequencingdatacontainscompletetranscriptomeofcodingandnoncodingRNAspecies
- Goodforintactandpartiallydegradedsmallsamplequantities–only1μgoftotalRNArequired
- VirtuallyallrRNAisdepletedfromintactandpartiallydegradedsamplesin2hours.
Sequencingisanimportantdiscoverytoolforplantresearch.ThesequencingdatafromsamplestreatedwithRibo-Zero(PlantLeaf)andRibo-Zero(PlantSeed/Root)isfocusedonvaluableRNAspecies,tofacilitatediscoveryofrelevantgenes,splicevariants,andisoforms.
Ribo-Zero(PlantLeaf)andRibo-Zero(PlantSeed/Root)offerinformativesequencingresultsbyremovingunwantedcytoplasmic,mitochondrial,andchloroplastrRNApriortosequencing.InordertoachievemaximumdepletionofrRNA,theRibo-Zero(PlantLeaf)kitcontainsahigherpercentageofprobestodepletechloroplastrRNAcomparedtoRibo-Zero(PlantSeed/Root).
OverviewoftheRibo-Zeroprocess
VirtuallyallrRNAisdepletedin2hours.
Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation
Alternativekits
ScriptSeqCompleteKit(Plant):Recommendedforlowinputplantsamples(e.g.100ngoftotalRNA).
Relatedinformation
rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs