• Removescytoplasmic,mitochondrial,andchloroplastribosomalRNA(rRNA)inasinglepass
• SequencingdatacontainscompletetranscriptomeofcodingandnoncodingRNAspecies
• Goodforintactandpartiallydegradedsmallsamplequantities–only1μgoftotalRNArequired
• VirtuallyallrRNAisdepletedfromintactandpartiallydegradedsamplesin2hours.

Sequencingisanimportantdiscoverytoolforplantresearch.ThesequencingdatafromsamplestreatedwithRibo-Zero(PlantLeaf)andRibo-Zero(PlantSeed/Root)isfocusedonvaluableRNAspecies,tofacilitatediscoveryofrelevantgenes,splicevariants,andisoforms.

Ribo-Zero(PlantLeaf)andRibo-Zero(PlantSeed/Root)offerinformativesequencingresultsbyremovingunwantedcytoplasmic,mitochondrial,andchloroplastrRNApriortosequencing.InordertoachievemaximumdepletionofrRNA,theRibo-Zero(PlantLeaf)kitcontainsahigherpercentageofprobestodepletechloroplastrRNAcomparedtoRibo-Zero(PlantSeed/Root).

OverviewoftheRibo-Zeroprocess

VirtuallyallrRNAisdepletedin2hours.

Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation

Alternativekits

ScriptSeqCompleteKit(Plant):Recommendedforlowinputplantsamples(e.g.100ngoftotalRNA).

Relatedinformation

rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs