ProductHighlights:
- RemovesribosomalRNA(rRNA)inasinglepass
- SequencingdatacontainscompletetranscriptomeofcodingandnoncodingRNAspecies
- Goodforintactandpartiallydegradedsmallsamples–only1μgoftotalRNArequired
Sequencingisanimportantdiscoverytoolforresearchintodisease,development,andmore.ThesequencingdatafromsamplestreatedwithRibo-Zero(Human/Mouse/Rat)isfocusedonvaluableRNAspecies,tofacilitatediscoveryofrelevantgenes,splicevariantsandisoforms.
Ribo-Zero(Human/Mouse/Rat)offersinformativesequencingresultsbyremovingunwantedrRNApriortosequencing.Thiskitremovescytoplasmic(nuclear-encoded)rRNAs.
Ribo-Zeroretainsthecompletetranscriptome
Ribo-ZeroremovesrRNAinordertoretaintheregulatoryregionsofnoncodingRNA,evenfromlow-qualitysamples.Othermethods,suchasPoly(A)depletion,donotretainnoncoding,regulatoryinformation.
OverviewoftheRibo-Zeroprocess
VirtuallyallrRNAisdepletedfromintact,degraded,andbadlydegraded(FFPE)samplesin2hours.
Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation
Alternativekitversions
TheRibo-ZeroGold(Human/Mouse/Rat)rRNARemovalKitremovesbothcytoplasmicandmitochondrialrRNAs,andiscompatIBLewithlowinputsamples(e.g.100ngoftotalRNA).
Relatedinformation
rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs
