ProductHighlights:
- RemoveribosomalRNA(rRNA)fromintactandpartiallydegradedGram(-)bacteria
- SequencingdatacontaincompletetranscriptomeofcodingandnoncodingRNAspecies
- Goodforsmallsamplequantities–only1μgoftotalRNArequired
- NuclearrRNAremoved:23S,16S,5S
Sequencingisanimportantdiscoverytoolforresearchintodisease,development,andmore.ThesequencingdatafromsamplestreatedwiththeRibo-ZerorRNARemovalKit(Gram-NegativeBacteria)arefocusedonvaluableRNAspecies,tofacilitatediscoveryofrelevantgenes,splicevariants,andisoforms.
TheRibo-ZerorRNARemovalKit(Gram-NegativeBacteria)offersinformativesequencingresultsbyremovingunwantedrRNApriortosequencing.ThekitremovesrRNAfromintactandpartiallydegradedsamples.
RemovesallmajorrRNAsubunits
TheRibo-ZerorRNARemovalKit(Gram-NegativeBacteria)depletesunwantedrRNAfromtotalRNA.RemovaloftheseunwantedsubunitsproducessequencingresultsthatareheavilyfocusedonBIOLOGicallyinformativegenomicregions.
OverviewoftheRibo-Zeroprocess
VirtuallyallrRNAisdepletedin2hours.
Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation
Alternativekitversions
TheRibo-ZerorRNARemovalKit(Bacteria)isrecommendedforsamplescomposedofmixturesofGram-negativeandGram-positivebacteria.
Relatedinformation
rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs
