ProductHighlights:
- RemovesribosomalRNA(rRNA)fromintactandpartiallydegradedmixturesofGram(+)andGram(–)bacteriainasinglepass
- SequencingdatacontainscompletetranscriptomeofcodingandnoncodingRNAspecies
- Goodforsmallsamplequantities–only1μgoftotalRNArequired
- VirtuallyallrRNAisdepletedfromintactandpartiallydegradedsamplesin2hours.
Sequencingisanimportantdiscoverytoolforresearchintodisease,development,andmore.ThesequencingdatafromsamplestreatedwiththeRibo-ZerorRNARemovalKit(Bacteria)isfocusedonvaluableRNAspecies,tofacilitatediscoveryofrelevantgenes,splicevariantsandisoforms.
Thiskitoffersinformativesequencingresultsbyremovingunwantedcytoplasmic(nuclear-encoded)rRNAfrombacterialmixturespriortosequencing.
OverviewoftheRibo-Zeroprocess
VirtuallyallrRNAisdepletedin2hours.
Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation
Alternativekits
ScriptSeqCompleteKit(Bacteria):Recommendedforsequencinglowinputsamples(e.g.100ngoftotalRNA).
Relatedinformation
rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs