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ProductHighlights:
- DepletesribosomalRNA(rRNA)
- SequencingresultscontainentiretranscriptomeofcodingandnoncodingRNAspecies
- Goodforintactandpartiallydegradedsmallsamples–only1μgoftotalRNArequired
Theyeasttranscriptomeismorecomplexthanpreviouslythought.RNA-Seqofyeastisavaluableapproachformappingthetranscriptometocharacterizenovelandlowabundancetranscripts.TheRibo-ZeroGoldrRNARemovalKit(Yeast)offersinformativesequencingresultsbyremovingunwantedrRNAbeforesequencing.
GenecoverageisenhancedfollowingdepletionbyRibo-ZeroGold(Yeast).Subsequentlibraryconstructionandsequencingproducesreadsfocusedoninformativegenomicregions.
OverviewoftheRibo-Zeroprocess
VirtuallyallrRNAisdepletedin2hours.
Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation
RelatedInformation
rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs