ProductHighlights:
- RemovesribosomalRNA(rRNA)inasinglepass
- SequencingdatacontainscompletetranscriptomeofcodingandnoncodingRNAspecies
- Goodforintactandpartiallydegradedsmallsamplequantities–only1μgoftotalRNArequired
Sequencingisanimportantdiscoverytoolforresearchintodisease,development,andmore.ThesequencingdatafromsamplestreatedwithRibo-ZeroGold(Human/Mouse/Rat)isfocusedonvaluableRNAspecies,tofacilitatediscoveryofrelevantgenes,splicevariants,andisoforms.
Ribo-ZeroGold(Human/Mouse/Rat)offersinformativesequencingresultsbyremovingunwantedrRNApriortosequencing.Thiskitremovesbothcytoplasmic(nuclear-encoded)andmitochondrialrRNAs.
Ribo-Zeroretainsthecompletetranscriptome
Ribo-ZeroremovesrRNAinordertoretaintheregulatoryregionsofnoncodingRNA,evenfromlow-qualitysamples.Othermethods,suchasPoly(A)depletion,donotretainnoncoding,regulatoryinformation.
OverviewoftheRibo-Zeroprocess
VirtuallyallrRNAisdepletedfromintact,degraded,andbadlydegraded(FFPE)samplesin2hours.
Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation
Alternativekits
Ribo-ZerorRNARemovalKit(Human/Mouse/Rat):RemovescytoplasmicrRNAonly.
Relatedinformation
rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs
Specifications:
| Technology | Sequencing |
| SpecializedSampleTypes | FFPE |
| SpeciesCategory | Human,Mouse,Rat |
| SystemCompatibility | MiSeq,GenomeAnalyzerIIx,HiSeq2000,NextSeq550,HiSeq3000,HiSeq1000,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |
