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ProductHighlights:
- RemovescytoplasmicandmitochondrialribosomalRNA(rRNA)fromsamplescomposedofhuman/mouse/rat,Gram-positiveandGram-negativebacterialRNA(seetable)
- Requires500ngto2.5μgtotalRNApersample
- rRNAremovalfrombothintactandpartiallydegradedRNA
- Single-pass,90-to120-minuteprocess
- Recoversallfragmentsofhumanandbacterialnon-ribosomalRNAs
- Applicabletoabroadspectrumofbacteria
TheRibo-ZeroGoldrRNARemovalKit(Epidemiology)combinesRibo-ZeroGold(Human/Mouse/Rat)andRibo-Zero(Bacteria)rRNAremovalreagentsintoasinglesolutionthateffectivelyremovesrRNA,includinghumanmitochondrialRNA,fromsamplescomposedofbothhuman/mouse/ratandbacterialRNA.
Hypothesis-freeNGSismoresensitivetodiversitythan16Sprofiling
Ribo-ZeroGoldrRNARemovalKit(Epidemiology)hasincreasedsensitivitytoBIOLOGicaldiversitybecauseitcapturesallmetatranscriptomicinformation(insteadofdirectedcapturewith16Sprofiling).
OverviewoftheRibo-Zeroprocess
VirtuallyallrRNAisdepletedin2hours.
Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation
RelatedInformation
rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs
Specifications:
Technology | Sequencing |
SpeciesCategory | Human,Mouse,Rat,Bacteria |
SpecializedSampleTypes | FFPE |
SystemCompatibility | GenomeAnalyzerIIx,HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |