ProductHighlights:
- CompleteViewoftheCFTRGene
Captureallvariantsintheproteincodingregionsandintron/exonboundariesoftheCFTRgene - AccurateResults
Deepcoverage(>3,000×)allowsdetectionaccuracywithaPositiveAgreement(PA)of99.66%* - NoDemographicBias
SequencingtheCFTRgeneremovesthebiasinherentinexistinggenotypingpanels1
*PAincludespolyTG/polyTvariants
TheMiSeqDxCysticFibrosisClinicalSequencingAssayisthefirstFDA-clearedinvitrodiagnostic(IVD)next-generationsequencing(NGS)testdesignedtosequenceallproteincodingregionsandintron/exonboundariesoftheCFTRgene,includingtwolargedeletions,twodeepintronicmutations,andindelsinhomopolymericregionssuchasthe2184delAdeletion.TheassayalsoautomaticallydetectspolyTG/polyTvariants.ViewingtheCFTRsequenceeliminatesanybiasinherentinexistingcysticfibrosis(CF)panels.Asaresult,theMiSeqDxCysticFibrosisClinicalSequencingAssaycanuncoverraremutationsnotaccountedforinstandardtests.
Widelyadoptednext-generationsequencing
TheMiSeqDxCysticFibrosisClinicalSequencingAssayprovidesafullyintegratedmolecularCFtestingsolutionontheMiSeqDxinstrument.Amplify27CFTRgenetargetregionsinasinglePCRstep,andmultiplexsamplestostreamlineyourworkflow.Simplypreparelibrariesfrom250nggDNA,loadontotheMiSeqDxforsequencingusingtheIlluminasequencingbysynthesis(SBS)chemistry,andanalyzewiththeuser-friendlysoftware.
Acompletekitforlibrarypreparationandsequencing
TheMiSeqDxCysticFibrosisClinicalSequencingAssayincludesallindexprimersandreagentsneededforlibrarypreparation,samplemultiplexing,andsequencinginasinglekit.MiSeqDxsequencingreagentsarepackagedinaconvenientready-to-useformat,minimizinghands-ontime,andincreasinguniformityinalltests.
Specifications:
| AssayTime | 2.5days |
| Hands-OnTime | 3.5hours |
| InputQuantity | 250nggenomicDNA |
| SystemCompatibility | MiSeqDx |
| Technology | Sequencing |
