ProductHighlights:
TheTruSightRNAPan-Cancerpanelprovidesacomprehensiveanalysisofthecancertranscriptome.Itoffers:
- Geneexpressioninformation,variantcalling,andfusiondetectionwithknownandnovelgenefusionpartners
- Optimized,low-inputprotocolforawiderangeofsampletypesincludingFFPE
- Acomprehensiveviewofcancerpathways
- EconomicalRNAsequencing(RNA-Seq)onadesktopsequencer
HighlySensitiveandEconomicalTargetedSequencing
TheTruSightRNAPan-Cancerpanelenablesthequantitativemeasurementofgeneexpressionaswellasthedetectionofgenefusionswithbothknownandnovelgenefusionpartners.Thepanelaccommodatesaslittleas10ngoftotalRNAinput(or20ngfromFFPEsamples).ThefocusonasubsetofrelevantgenesenablesRNA-Seqwithhighsensitivityat8samplesperrunonadesktopsequencer,allowingcost-effectiveaccesstoNGSforanylab.
IntuitiveCloud-basedDataAnalysis
AnalysiscanbeperformedusingtheRNA-SeqAlignmentBaseSpaceApp.Thisintuitivetoolperformsfusioncallingandvariantdetection,andprovidesgeneexpressionprofiles,offeringacomprehensivesolutiontoallcancerresearchers.
TruSightRNAPan-CancerSampleDatasets
MiSeqData
10ngofhumanreferenceRNA(UHR),humanreferencebrainRNA(Brain),celllineRNA(MCF7),and20ngofbreasttumorFFPERNA(BT)werepreparedusingtheTruSightRNAPan-CancerPanelandsequencedontheMiSeqSystem.ReadmappingandfusioncallingwereperformedusingtheRNA-SeqAlignmentAppwithSTARaligneronBaseSpaceSequenceHub.
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MiniSeqData
HumanreferenceRNA(HBRRandUHRR),celllineRNA(MCF7),andbreasttumorRNAsampleswerepreparedusingtheTruSightRNAPan-Cancerpanel,and8samplesweresequencedontheMiniSeqSystemata2x76bpreadlengthwithsingleindexing.Thetotalyieldwas4.2Gbwith96.8%ofbasesatoraboveQ30.
BrowsethedatainBaseSpaceSequenceHub:
- ViewRun
- ViewProject
AccesstothisdatarequiresaBaseSpaceSequenceHublogin.
RegisterforBaseSpaceSequenceHub
Specifications:
| AssayTime | 2.5days |
| Hands-OnTime | 11hours |
| InputQuantity | 10ngtotalRNA,20-100ngFFPERNA |
| SystemCompatibility | MiSeq,NextSeq550,MiSeqDxinResearchMode,MiniSeq,NextSeq500 |
| Method | TargetedRNASequencing,SequencingforCytogenomics |
| VariantClass | GeneFusions,SomaticVariants,NovelTranscripts,TranscriptVariants |
| SpecializedSampleTypes | FFPE,LowInput |
| Technology | Sequencing |
| SpeciesCategory | Human |
| CancerType | Pan-Cancer |
