TheTruSightHLAv2SequencingPanelprovidesclinicalresearcherswithabroad-coverage,ultra-high–resolutionhumanleukocyteantigen(HLA)typingsolutionforsimple,rapidassessmentoftheHLAregioninasingleassay.Itfeatures:

• High-resolutionsequencingof11HLAloci
• Workflowwithlibrarypreparation,sequencinganddataanalysis/reporting
• Accurateandhigh-resolutionHLAtypingforunambiguousresults

CaptureFullHLAGeneSequences

TruSightHLAv2coversallcommonlytypedHLAloci,plusthosewithemergingrelevance.ThispanelenablessequencingofClassIHLA-A,B,andC;ClassIIHLA-DRB1/3/4/5,HLA-DQA1,HLA-DQB1,HLA-DPA1,andHLA-DPB1.Thisexpandscoveragebeyondtheclassicloci,withfullgenecoverage,enablingdiscoveryofnewalleleswithouttheneedtodesignnewprimers.

Sample-to-ReportWorkflowwithOptimizedDataAnalysis

ThecomprehensivesolutionincludesreagentsandsoftwareoptimizedforHLAanalysis.Asimplifiedworkflowenablesturnaroundtimeoflessthan48hoursandincreasedefficiencywithlessthan4.5hoursofhands-ontime.On-instrumentsoftwareanalyzessequencedata;TruSightHLAAssignSoftwareprovidesflexIBLeoptionsforpost-analysisreportingofresults.

High-AccuracyHLATyping

TheexpandedcoverageofTruSightHLAv2provideshighresolution,eliminatingtheneedforfollow-uptestingtoobtainaconfidenttypingresult.ViewHLALoci.

HowTruSightHLAv2Works

TruSightHLAv2amplifiesHLAlociwithlong-rangePCR.Afteramplification,NexteratagmentationfragmentstheDNAandleavesbehindasequencetag.AnadditionalPCRthenaddssequenceadaptersandindexingprimerstogeneratesequencing-readyDNAlibraries.PreparedlibrariesarethenloadeddirectlyontoaMiniSeq,MiSeqorNextSeqSystemforsequencing.

AnalysisSoftwareHighlights

TheAssign2.0TruSightHLAanalysissoftwareassistswiththeassignmentofahumanleukocyteantigen(HLA)type.ThesoftwareisdesignedtoanalyzedatafromlibrariespreparedwithIlluminaTruSightHLASequencingPanelsandthensequencedonanIlluminasequencer.UseAssign2.0toimportsequencedata,performbasecalling,editsequences,andcompareasampleconsensussequencewiththeIMGT/HLAdatabaseofalleles.