ProductHighlights:
TruSeqTargetedRNAExpressionKitsenablehighlycustomizablemid-tohigh-plexgeneexpressionprofilingstudies.
Youcandefinepanelsof12-1,000assaystotargetindividualexons,isoforms,splicejunctions,codingSNPS(cSNPs),genefusions,andnon-codingRNAtranscripts,plusmultiplexupto384samples.Generatethedataequivalentofupto25,000individualRT-PCRassaysinasinglerun.*
Assaysincludeconvenientonlinepaneldesignandordering,arapidandsimpleworkflowstartingwithaslittleas50ngofRNA,andautomated,on-instrumentdataanalysis.
TypesofStudyDesigns
- Custompanels—selectupto1000targetsfromadatabaseofover400,000predesignedassays.
- Add-on(supplemental)content—addasecondoligopoolofupto1000targetstoapreviouslydesignedTruSeqTargetedRNAExpressioncustompanel.
StartaCustomProject
OrdercustompanelsusingDesignStudio,afree,user-friendly,web-basedtool.Simplyselecttheassaysyouneedandaddthemtoyourorder,withnodesigntime.
LogintoDesignStudio
BrowseTruSeqTargetedRNASampleData
HumanBrainReferenceRNA(HBRR),UniversalHumanReferenceRNA(UHRR),andhumanliverRNAsampleswerepreparedusingtheTruSeqTargetedRNAExpressionKit.
TheselibrariesweresequencedontheMiniSeqSystemusingtheMiniSeqHighOutputReagentKitata1x51bpreadlengthconfiguration.Thetotalyieldwas2.04 Gbwith96.5%ofbasesatoraboveQ30.
NotethataccesstothisdatarequiresaBaseSpaceSequenceHublogin.
RegisterforBaseSpaceSequenceHub
ViewTruSeqTargetedRNAProjectData
(secondaryanalysiswithBaseSpaceApps)
*ReferstoinstrumentswithcatalognumberSY-410-1001andupgrade,orcatalognumberSY-410-1003
Specifications:
| AssayTime | 1day |
| Hands-OnTime | 4hours |
| InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
| ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
| Multiplexing | Upto384samplespersequencingrun |
| MechanismofAction | Amplification |
| Method | TargetedRNASequencing |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,TranscriptVariants |
| SpecializedSampleTypes | FFPE |
