ProductHighlights:
TheTruSeqStrandedmRNALibraryPrepKitforNeoPrepoffersastreamlined,cost-efficient,andscalablesolutionforcodingtranscriptomeanalysis.Itenablesrobustinterrogationofbothstandardandlow-qualitysamples.
- Simplifiedandseamlessworkflowsolution–Generatelibrarieswithminimalhands-ontime
- Unparalleledperformanceandreproducibility–InterrogategeneexpressionwithTruSeqStrandedmRNAcoverageandquality,andreduceduservariABIlity
- Lowinputrequirement–Beginlibraryprepwithaslittleas25ngtotalRNA
PreciseandAccurate
GetprecisemeasurementofmRNAstrandorientationfordetectionofantisensetranscription,enhancedtranscriptannotation,andincreasedalignmentefficiency.Highcoverageuniformityenhancesthediscoveryoffeaturessuchasalternativetranscripts,genefusions,andallele-specificexpression.
Cost-Efficient
Strandedinformationidentifiesfromwhichofthe2DNAstrandsagivenRNAtranscriptwasderived.Thisinformationprovidesincreasedconfidenceintranscriptannotation,particularlyfornonhumansamples.Identifyingstrandoriginincreasesthepercentageofreadsthatalign,reducingsequencingcostspersample.
NeoPrepSystemenhancesTruSeqStrandedmRNAperformance
TheNeoPrepSystemenhancesTruSeqStrandedmRNAperformancebyprovidinghigh-qualityreproducIBLeresults,evenwithlowinputamountsofRNA.Digitalmicrofluidicstechnologypreciselymanipulatesdropletsthatperformthelibraryprepworkflow,withinthetightlycontrolledenvironmentoftheNeoPreplibrarycard.
Asimple,intuitiveworkflowdelivers16libraries,eliminatingmostmanualsteps,andreducinghands-ontimefrom~4.5hourstojust30minutes.Inaddition,digitalmicrofluidicsrequireslessRNAinput,enablingexcellentperformancefromaslittleas25ngoftotalRNA.
Specifications:
| AssayTime | ~10.5hours |
| Hands-OnTime | ~30minutes |
| InputQuantity | 25to100ngtotalRNAfromspecieswithpolyAtails |
| ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
| MechanismofAction | Oligo-dTbeadscapturepolyAtails |
| Multiplexing | Upto24-plexpoolingwithadditionalNeoPrepruns |
| SampleThroughput | Upto16samplesperNeoPreplibrarycard |
| SystemCompatibility | GenomeAnalyzerIIx,HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,NeoPrep,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |
| SpeciesCategory | Other,Mammalian,Bovine,Mouse,Human,Rat |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,NovelTranscripts,TranscriptVariants |
| Technology | Sequencing |
| Method | mRNASequencing |
| AutomationCapability | NeoPrepDigitalMicrofluidics |
