ProductHighlights:
High-QualityWhole-TranscriptomeAnalysiswithPreciseStrandInformation
RNA-Seqtechnologyprovidesuniformcoverage,precisemeasurementofstrandorientationandhigh-confidencediscoveryoffeaturessuchasalternativetranscripts,antisenseexpressionandallele-specificexpressionacrossbothcodingandmultipleformsofnoncodingRNA.TheTruSeqStrandedTotalRNAwithRibo-ZeroPlantKitcouplesthebenefitsofTruSeqRNAlibrarypreparationwithRibo-ZeroribosomalRNAreductionchemistry,providingarobustandscalablesolutionforwhole-transcriptomeanalysis.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
EfficientRibosomalRNAReductionAcrossaRangeofPlantSpecies,StudyDesigns
TheTruSeqStrandedTotalRNAwithRibo-ZeroPlantKitenablesrapidandspecificremovalofcytoplasmic,mitochondrialandchloroplastribosomalRNAfromleaf,seed,androottissue.Thekithasbeenvalidatedforuseinmultipleplantspecies,includingArABIdopsisthaliana,rice,andmaize,andwillprovideefficientrRNAremovalinabroadrangeofadditionalspecies.*
*Forinformationonaparticularspeciesofinterest,contactIlluminaTechnicalSupport.
Specifications:
| AssayTime | ~6.5hours |
| Hands-OnTime | ~3.5hours |
| InputQuantity | 10nghigh-qualitygenomicDNA;10–100ngFFPEDNA(dependingonQCresults) |
| MechanismofAction | Probehybridization,extension-ligation,andPCR |
| Multiplexing | 1–96 |
| ContentSpecifications | Designcustomprobestosequencegenomicregionsofinterest.Contentrange:4–650kb |
| VariantClass | ShortTandemRepeats(STRs),SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels) |
| SystemCompatibility | HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq1500,NextSeq500,MiSeqFGxinResearchMode,HiSeq2500,HiSeq4000 |
| SpeciesCategory | Other,Bovine,Mouse,Human,Rat |
| SpecializedSampleTypes | FFPE,LowInput |
| Technology | Sequencing |
| AutomationCapability | LiquidHandlingRobots |
| Method | AmpliconSequencing,CustomSequencing |
