ProductHighlights:
TruSeqStrandedTotalRNAwithRibo-ZeroGlobinkitsdeliveraclearandcomprehensiveviewofthetranscriptomefromblood-derivedRNA,withafast,efficientsequencinglibrarypreparationworkflow.
High-qualitywhole-transcriptomeanalysiswithprecisestrandinformation
ThesekitscouplethebenefitsofRibo-ZeroribosomalRNAreductionchemistrywithRNAsequencing(RNA-Seq)technologyforwhole-transcriptomeanalysisofhuman,mouse,orratsamples.Thistechnologyprovides:
- Uniformcoverageandprecisemeasurementofstrandorientation
- High-confidencediscoveryofalternativetranscripts,antisenseexpression,andallele-specificexpression
- Discoveryacrossbothcodingandmultipleformsofnon-codingRNA
EfficientribosomalRNAandglobinmRNAremovalinasinglestep
Whole-transcriptomeanalysisofblood-derivedRNArequirestheremovaloftwoformsofabundantRNA—ribosomalRNA(bothcytoplasmicandmitochondrial)aswellasglobinmRNA,whichispresentinhighlevelsinwholeblood.
TrADItionalremovalmethodsrequiretwoindependentsteps.Thismeanstheneedforadditionalreagents,alongerworkflow,andmoreinputRNAlost.TruSeqStrandedTotalRNAwithRibo-ZeroGlobinleveragesRibo-ZerochemistrytoefficientlyremovebothformsofabundantRNAinasingle,rapidstep.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
InputQuantity | 0.1–1ughigh-qualitypurifiedtotalRNAfromblood |
ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
MechanismofAction | Bead-basedrRNAdepletion,CDNAsynthesis,andPCR |
Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkitversion:Prepare96uniquelyindexedsamples |
SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq500,HiSeq2500,NextSeq550,HiSeq3000,HiSeq1000 |
AutomationCapABIlity | LiquidHandlingRobots |
VariantClass | TranscriptVariants,SingleNucleotidePolymorphisms(SNPs),GeneFusions |
SpeciesCategory | Rat,Mouse |
Method | Whole-TranscriptomeSequencing |
Technology | Sequencing |