ProductHighlights:
TruSeqSmallRNALibraryPreparationKitsprovidereagentstogeneratesmallRNAlibrariesdirectlyfromtotalRNA.MicroRNAs(miRNAs)generatedbyDicerprocessingareefficientlytargetedbytheincludedmodifiedadapters.
Thesekitsenablemultiplexedsequencingwiththeintroductionof48uniqueindexes,allowingmiRNAandsmallRNAdiscoveryandprofilingthroughputtomatchtheunparalleledoutputofIlluminasequencing.Indexesareaddedinauniversalamplificationreaction,greatlyreducingligationbiasandensuringaccuratemeasurementofmiRNAexpression.
Workflowimprovementsenablestreamlinedsamplepreparation,allowingeconomicalstudiescoveringallsmallRNAtranscriptsinanyspecies.CompatIBLeapplicationsincludefindingnovelmiRNAs,characterizingvariationsuchasisomerswithsingle-baseresolution,andanalyzingdifferentialexpressionwithoutpriorassumptions.
TruSeqSmallRNASampleDatasets
TheHumanBrainReferenceRNA(HBRR)andUniversalHumanReferenceRNA(UHRR)sampleswerepreparedusingtheTruSeqSmallRNALibraryPrepKit.TheselibrariesweresequencedontheMiniSeqSystemusingtheMiniSeqHighOutputReagentKitata1x36bpreadlengthconfiguration.Thetotalyieldwas0.91Gbwith97%ofbasesatoraboveQ30.
BrowsetheDataInBaseSpaceSequenceHub:
- ViewRun(primaryanalysisandmetrics)
- ViewProject(secondaryanalysiswithBaseSpaceApps)
Specifications:
| AssayTime | ~10.5hours |
| Hands-OnTime | ~30minutes |
| InputQuantity | 25to100ngtotalRNAfromspecieswithpolyAtails |
| ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
| MechanismofAction | Oligo-dTbeadscapturepolyAtails |
| Multiplexing | Upto24-plexpoolingwithadditionalNeoPrepruns |
| SampleThroughput | Upto16samplesperNeoPreplibrarycard |
| SpeciesCategory | Drosophila,AnySpecies,Mouse,Zebrafish,Human,Rat,Plant,Nematode |
| SystemCompatibility | MiSeq,NextSeq550,MiniSeq,NextSeq500 |
| SpecializedSampleTypes | FFPE,LowInput |
| Technology | Sequencing |
| AutomationCapABIlity | LiquidHandlingRobots |
| VariantClass | NovelTranscripts,TranscriptVariants |
| Method | miRNAandSmallRNASequencing |
