ProductHighlights:
TheTruSeqRapidExomeLibraryPrepKitdeliversasimple,efficientmethodforhigh-confidencecallingofexonicvariants.Thefastlibrarypreparationandexomeenrichmentworkflowdeliverlibrariesin1daywithnoneedforadditionalequipment,givinglabstheflexibilitytoplanprojectsaccordingtosamplevolume.Thiskitoffers:
- Enhancedtransposomechemistryresultsinreducedbias,providingconsistentlibrarypreparationandexomeenrichment.
- Rapid,automation-friendlyworkflowcompletesin1daywithonly3hoursofhands-ontime.
- Highcoverageuniformityacrossexonicregionsandon-target%
ProvenTruSeqDataQuality
TheTruSeqRapidExomeLibraryPrepKitdeliversanaverageof75%ofon-targetsequencingreads.Thishighon-targetpercentagerequiresfewersequencingcyclestoreachdesiredcoveragelevels,butstillachievesuniformcoverageforhigh-confidenceresults.
Tolearnmoreaboutcalculatingcoverageestimates,seethesequencingcoveragecalculator.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
AssayTime | 1day |
Hands-OnTime | 3hours |
InputQuantity | 50nggenomicDNA |
ContentSpecifications | Capturescodingexons.Covers45Mbofexoniccontent(≥98%ofRefSeq,CCDS,andEnsemblcodingcontent). |
MechanismofAction | Transposase-basedfragmentationandexomeenrichmentwithbiotinylatedcaptureprobes |
Multiplexing | Upto12-plexenrichment |
Technology | Sequencing |
SpeciesCategory | Human |
AutomationCapABIlity | LiquidHandlingRobots |
SystemCompatibility | NextSeq500,NovaSeq6000,HiSeq4000 |
Method | ExomeSequencing |