ProductHighlights:
TruSeqExomeLibraryPrepKitisacost-effectivelibrarypreparationandexomeenrichmentsolutionwithindustry-leADIngaccuracy.Itoffers:
- Pre-enrichmentlibrarypoolingandoptimalcoverageforlow-costexomesequencing
- MechanicalshearingandTruSeqenrichmenttechnologythatyielduniformcoverageandgreaterthanorequalto80%on-targetsequencingreads
- Fullysupportedworkflowsolutiontosimplifyexomesequencing
Cost-EffectiveExomeSequencing
TheTruSeqExomeLibraryPrepKitsupports12-plexpre-enrichmentlibrarypooling,enablingresearcherstomaximizesequencingthroughputandidentifyvariantsinlesstimebysequencingupto12librariesperflowcelllane.Thehighon-targetpercentagerequiresfewersequencingcyclestoreachtheoptimallevelofcoverage,whilestillachievinghighcoverageuniformityforconfidentresults.Italsoenablessequencingofmoreexomesperrun,soresearcherscanmaximizetheirbudgets.
Tolearnmoreaboutcalculatingcoverageestimates,seethesequencingcoveragecalculator.
ProvenTruSeqDataQuality
TheTruSeqExomeLibraryPrepKitdelivers≥80%ofon-targetsequencingreadsforefficient,cost-effectiveexomesequencing.BycombiningtheTruSeqExomeLibraryPrepKitwithIlluminasystemsusingsequencingbysynthesis(SBS)technology,researcherscanidentifytruecodingvariantsandminimizefalse-positiveandfalse-negativecalls.
Convenient,IntegratedWorkflowSolution
Illuminaprovidesanintegrated,fullysupportedworkflowsolutionthatguidesresearchersfromlibrarypreparationthroughanalysis.Allcomponentsoftheexomesequencingworkflowaredesigned,optimized,andanalyticallyvalidatedtogether.ExpertIlluminascientistsprovideasinglesourceoftechnicalandfieldsupportforeverystepintheprocess.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
| AssayTime | 2.5days |
| Hands-OnTime | 6hours |
| InputQuantity | 100nggenomicDNA |
| ContentSpecifications | Capturescodingexons.Covers45Mbofexoniccontent(≥98%ofRefSeq,CCDS,andEnsemblcodingcontent). |
| MechanismofAction | Mechanicalfragmentation(COVARIS)andexomeenrichmentwithbiotinylatedcaptureprobes |
| Multiplexing | Upto12-plexenrichment |
| SystemCompatibility | NovaSeq5000,NextSeq550,HiSeq3000,NextSeq500,NovaSeq6000,HiSeq4000 |
| SpecializedSampleTypes | FFPE |
| Technology | Sequencing |
| SpeciesCategory | Human |
| AutomationCapABIlity | LiquidHandlingRobots |
| Method | ExomeSequencing |
