ProductHighlights:
TruSeqDNAPCR-FreeLibraryPreparationKitsprovidesimple,all-inclusivelibrarypreparationforwhole-genomesequencingapplications.Researcherscansequenceawidevarietyoforganisms,fromsmallgenomessuchasbacteriatohumanwhole-genomes.Thekitsoffer:
- Shortenedgel-freeworkflowsthatremovetheneedforPCR
- ABIlitytosequencethemostchallengingregions
- Improvedgenomecoveragetoidentifythegreatestnumberofvariants
Sequencethemostchallengingregions
TruSeqDNAPCR-freekitsoffersuperiorcoverageofareaswhicharetrADItionallydifficulttosequencesuchashighGC-richregions,promoters,andrepetitivecontent.
ThekitsaretunabletoavarietyofreadlengthsandaresupportedonallIlluminasequencinginstruments.Thispermitstheresearchertotailoreachruntotheneedsoftheexperiment.
Detectthegreatestnumberofvariants
PCR-freemeansreducedlibrarybiasandgaps.Theresultisunsurpasseddataquality.Thisenablesyoutodetectthegreatestnumberofvariants.ExcellentgenomecoveragemeansyourresultshavethelowestandsmallestnumberofgapsandenhancedcoverageofhighG/Crichregions.
UsePCR-freeforfasterprotocols
RemovingPCRcreatesafasterprotocolandsuperiordataquality.Bead-basedsizeselectionshortenstheworkflow.IntandemwithIlluminasequencingsystems,theTruSeqDNAPCR-FreeLibraryPreparationKitprovidesarangeofenhancementstotheindustry"smostwidelyadoptedlibrarypreparationworkflow.
AccessflexIBLethroughputoptions
Kitsincludereagents,samplepurificationbeads,andindexes,withtwooptionsforflexibility:
- TruSeqDNAPCR-FreeLTLibraryPreparationKitssupport24-plexmanualprocessingforlow-throughputstudies.
- TruSeqDNAPCR-FreeHTLibraryPreparationKitsare96-plexforhigh-throughputstudies,andcanbeautomatedonliquidhandlingrobots(orprocessedmanually).
Findanup-to-datelistofautomationvendorswithroboticsystemsthatsupporttheHTlibrarypreparationkits
Specifications:
| InputQuantity | 0.1–1ughigh-qualitypurifiedtotalRNAfromblood |
| ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
| MechanismofAction | Bead-basedrRNAdepletion,CDNAsynthesis,andPCR |
| Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkitversion:Prepare96uniquelyindexedsamples |
| SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq500,HiSeq2500,NextSeq550,HiSeq3000,HiSeq1000 |
| AutomationCapability | LiquidHandlingRobots |
| VariantClass | TranscriptVariants,SingleNucleotidePolymorphisms(SNPs),GeneFusions |
| SpeciesCategory | Rat,Mouse |
| Method | Whole-TranscriptomeSequencing |
| Technology | Sequencing |
