ProductHighlights:
- Unlocksmallsamples(50–100ngDNAinput)
- CpG,CHH,&CHGregionsincludedforcomprehensive,whole-genomeresults
- Fastprotocol–five-hourmethod
- Capturefullsamplediversity
Sequencetheentiresample–nolossofinformation
TheprocessofbisulfitetreatmentdenaturesgenomicDNAintosinglestrandedDNA.TruSeqDNAMethylationconvertssinglestrandedDNAintoanIlluminasequencinglibrary.AllssDNAfragmentsarecapturedintoanIlluminasequencinglibrary,thereforeeliminatingthelossofdiversityassociatedwithothermethods.
Supportedanalysisinthecloud
TruSeqDNAMethylationlibrariescanbealignedtothehumangenomeandcomparedfordifferentialmethylationwithIlluminaBaseSpaceAppsMethylSeqandMethylKit.TheseapplicationsarefullysupportedandweredevelopedspecificallyforTruSeqDNAMethylationlibrarypreparation.
ExampledatasetsforTruSeqDNAMethylationlibraries,alsoavailableinBaseSpaceSequenceHubDataCentral(usethe“MethylSeq”categoryfilter),demonstrateunparalleledqualityandseamlessanalysis.
Deepcoverageofcriticalgenomicregions
DepthofcoverageisenhancedingenomicareaswithBIOLOGicalutility.TruSeqDNAMethylationcapturesfullsamplediversityofcriticalareas,including:
- Codingregionstartandendforexonsfromthecanonicaltranscriptofproteincodinggenesforgenesknowntobeinvolvedincancer,takenfromSOMAandCRUKpanels(aswellasliterature-derivedcancergenes)
- GenesdefinedbytheAmericanCollegeofMedicalGeneticsasbeingmedicallyrelevant(ACMGgenes)
- ExoniccodingregionsfromEnsemble70
- Listof100promotersdefinedbytheBroadInstituteasbeingofhighinterestanddifficulttosequence