ProductHighlights:
TruSeqCustomAmpliconisafullycustomizable,amplicon-basedassayfortargetedresequencing.Theassayenablesyoutofocusinterrogationonkeyregionsofgenomicinterest.Upto1536ampliconscanbesequencedinasinglereactionusingasimpleworkflow.Thishighlytargetedapproachoffersunparalleledefficiencyfordiscovering,validating,andscreeninggeneticvariantsinareasofresearchfocus.
Requiringaslittleas50ngofinputgenomicDNA(gDNA),thiskitcanbeusedwithawiderangeofsampletypes.TheABIlitytomultiplexupto1536ampliconsperreactionallowscoverageofupto650kbofcumulativesequence.Further,anintegrateddualindexingschemesupportsupto96samplesperrun,enablingyoutofullybenefitfromdesktopsequenceroutputcapabilities.
ThisassayprovidesimprovedperformanceindifficulttoaddressGC-richregions,aswellasincreasedlibraryyield,uniformity,andstability.Additionally,optimizedamplificationstepsenablesampleinputrequirementstodropfrom150ngto50ng.
SimplifiedProbeDesign
DesigntargetedTruSeqCustomAmpliconoligonucleotideprobeswithDesignStudio,afree,easy-to-useonlinetoolthatprovidesoptimizedcoverage.
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SimpleDataAnalysis
AutomateddataanalysisusingtheBaseSpaceTruSeqAmpliconAppallowsresearcherstoeasilyreviewprojectdata,includingvariantsdetected,acrossmultipleruns.
SpeciesCompatibility
TruSeqCustomAmpliconsupportshuman,mouse,rat,andbovinesamples.DesignStudiodesignalgorithmsarealsoavailableformaize,rice,pig,dog,soybean,chicken,andsheep;however,assayperformancecannotbeguaranteedastheresultingassayshavenotbeentested.Inaddition,IlluminaConciergeservicescandesigncustomassaysand/orprovideassayoptimizationsupportforanyspeciesofinterest.
InquireaboutIlluminaConciergeservicesFindanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
| AssayTime | 1day |
| Hands-OnTime | 4hours |
| InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
| ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
| Multiplexing | Upto384samplespersequencingrun |
| MechanismofAction | Amplification |
| VariantClass | ShortTandemRepeats(STRs),SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels) |
| SystemCompatibility | HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq1500,NextSeq500,MiSeqFGxinResearchMode,HiSeq2500,HiSeq4000 |
| SpeciesCategory | Other,Bovine,Mouse,Human,Rat |
| SpecializedSampleTypes | FFPE |
| Technology | Sequencing |
| AutomationCapability | LiquidHandlingRobots |
| Method | AmpliconSequencing,CustomSequencing |
