ProductHighlights:
FastLibraryPrep,OptimizedforSmallGenomes,PCRAmplicons,andPlasmids
- Rapidlibrarypreparation–completelibraryprepinaslittleas90minuteswithonly15minutesofhands-ontime
- Fasttimetoresults–gofromDNAtodatain8hourswithourbenchtopsequencers.
- Optimizedforsmallgenomes,PCRamplicons,andplasmids–onelibraryprepkitformanyapplications
- Innovativesamplenormalization–eliminatestheneedforlibraryquantificationbeforesamplepoolingandsequencing
WithNexteratechnology,DNAissimultaneouslyfragmentedandtaggedwithsequencingadaptersinasingletubeenzymaticreaction.NexteraXTsupportsultra-lowDNAinputofonly1ng.Itenablesawiderangeofinputsamples,including smallgenomes,PCRampliconsgreaterthan300bp,plasmids,microbialgenomes,concatenatedamplicons,anddouble-strandedCDNA.
Multiplexingofupto384samplesperNexteraXTlibraryisalsoavailableforthoseprojectsrequiringgreaterthroughput.Plus,thekitincludesaninnovativebead-basedsamplenormalizationthateliminatestheneedforlibraryquantificationpriortopoolingandsequencing.LibrariespreparedwithNexteraXTkitsarecompatIBLewithallIlluminasequencers.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
Specifications:
AssayTime | 90minutes |
Hands-OnTime | 15minutes |
MechanismofAction | EnzymaticFragmentation |
Multiplexing | Upto384uniquelyindexedsamplesmaybepooledandsequencedtogether. |
InputQuantity | 1ngDNA |
SpeciesCategory | Drosophila,AnySpecies,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria |
SystemCompatibility | MiSeq,NextSeq550,NextSeq500,MiSeqFGxinResearchMode |
Method | ShotgunSequencing,Whole-GenomeSequencing,DeNovoSequencing,16SrRNASequencing,AmpliconSequencing,TargetedDNASequencing |
VariantClass | SingleNucleotidePolymorphisms(SNPs),StructuralVariants |
SpecializedSampleTypes | LowInput,SingleCells |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
NexteraXTLibraryPreparationWorkflowimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-1.png:
ThecombinationofNexteraXTandrapidsequencingwiththeMiSeqSystemprovidesacompleteDNAtodataworkflowinonly8hours.InnovativeSampleNormalizationimages/illumina/201710/nextera-xt-dna-library-prep-kit-web-graphic-2.pngIllumina-BovineHD DNA分析试剂盒这个全面的全基因组牛基因分型阵列试剂盒具有超过777,000个SNP,可与任何种类的牛肉或奶牛兼容。产品亮点BovineHD BeadChip是一个全面的全基因组牛基因分型阵列,可用于询问任何牛肉和奶牛品种的遗传变异。Illumina与包括USDA-ARS,UNCEIA-INRA,辉瑞动物遗传学和密苏里大学在内的主要牛农业思想领袖合作开发了该产品。该BeadChip具有超过777,000个SNP,它们均匀地分布在整个牛基因组中,可实现广泛的应用,例如全基因组选择,鉴定数量性状位点,评估遗传优势,杂交育种,连锁不平衡研究,比较遗传研究和品种鉴定以评估生物多样性。八样本的BovineHD BeadChip以及经过验证的Infinium HD分析法为牛的全基因组研究提供了强大的高通量解决方案。777,000 SNP BovineHD BeadChip支持全基因组范围内的全面基因分型研究,扩展了在遗传预测中评估的牛品种的多样性,并使更多的定量性状得以发现。技术指标输入数量200 ng DNA(50 ng / ul)标记数固定标记:777,962个在基因组中均匀分布的SNPs自定义标记附加能力:无分析类型Infinium HD Super方法全基因组基因分型阵列自动化能力自动阵列装载机,液体处理机器人系统相容性扫描仪种类类别牛的技术微阵列特殊样品类型FFPE纸巾核酸类型脱氧核糖核酸