Description:

Nanocs LipoPEG® Cholesterol PEG DBCO is a lipophilic PEG derivative with a DBCO reactive group. DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction. This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO PEG reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.

Physical Properties:

• Yellow/pale-yellow solid or semi-solid;
• Soluble in DMSO or DMF;

Storage Conditions:

• Store at -20 ⁰C, desiccate. Avoid frequent thaw and frozen.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

Nanocs代理        在生命科学领域，苏州蚂蚁淘生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝苏州蚂蚁淘生物正式成为Nanocs品牌修饰性PEG的签约代理商，在这里要感谢广大客户多年来对苏州蚂蚁淘生物科技有限公司的支持和厚爱，我们将一如既往的为广大客户带来Nanocs品牌修饰性PEG高品质的产品和服务，欢迎广大新老客户来电咨询。 anocs PEG官能团 PEG Reagents by Functional Group