- 单击化学试剂
- Amine Reactive PEGs
- Biotin PEG
- Nanoparticles & Beads
- 乙烯砜聚乙二醇
- Fluorescent PEGs
- NTA PEG
- NHS cross linkers
- ITO Coated Glass Slides
- Polylysine & conjugates
- Dextran & Conjugates
- 菁聚乙二醇
- Functional Agarose Beads
- Carbonyl Reactive PEGs
- 表面涂层功能性载玻片
- Fluorescein PEG
- 其他聚乙二醇试剂
- Phosphate PEG
- 蛋白质生物结合物
- NPC PEG
- Thiol PEG
Description:
MethoxyPEGpropionaldehydeisastablealdehydePEGthatcanbeusedforprotein,antibody,peptidesorcarbohydrateN-terminalaminegrouppegylationreagent.Thereactionbetweenaldehydegroupwiththeα-amineattheN-terminusproducesanintermediateSchiffbase.FurtherreductionwithhydridewillformastableC-Nbond.PEGaldehydesreactwithn-terminusaminegroupsatapHoffrom5.0to9.5.HigherpHwillresultinmultiplePegylationwithbothterminalandlysinegroups.
PhysicalProperties:
- Off-white/whitesolid;
- Solubleinwater,DMSO,DMF;
- Activity>90%;Peroxide<10ppm;>10ppm;>
StorageConditions:
- Storeat-200C
ReactionProcedures:
Generally,a5-to10-foldmolarexcessofPEGaldehydeovertheamountofamine-containingmaterialresultsinsufficientconjugation.
MaterialsRequired:
- Pegylationbuffer:Amine-freeaqueousbuffer,pH5.0~6.5.
- PEGaldehydestocksolution:50mgin1mLconjugationbuffer.
- Washingsolution:Distilledwateroranyaqueousbuffer.
ReactionSteps:
Dissolvetargetedmaterialsinpegylationbuffer.Estimatetheconcentrationofprimaryaminegroupsonthetargetedmaterials.AddPEGaldehydestocksolutiontothetargetedconjugationmaterialswiththefinalconcentrationkeepatleast10mg/mL.Allowmixtureagitatesatroomtemperaturefor2~4hrsatroomtemperatureorovernight40C.Afterreaction,C=Nbondcanbereducedbysodiumborohydride.Conjugatecanbepurifiedeitherbysizeexclusionchromatographyordialysis.
Nanocs代理 在生命科学领域,苏州蚂蚁淘生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝苏州蚂蚁淘生物正式成为Nanocs品牌修饰性PEG的签约代理商,在这里要感谢广大客户多年来对苏州蚂蚁淘生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Nanocs品牌修饰性PEG高品质的产品和服务,欢迎广大新老客户来电咨询。 anocs PEG官能团 PEG Reagents by Functional Group