- 单击化学试剂
- Amine Reactive PEGs
- Biotin PEG
- Nanoparticles & Beads
- 乙烯砜聚乙二醇
- Fluorescent PEGs
- NTA PEG
- NHS cross linkers
- ITO Coated Glass Slides
- Polylysine & conjugates
- Dextran & Conjugates
- 菁聚乙二醇
- Functional Agarose Beads
- Carbonyl Reactive PEGs
- 表面涂层功能性载玻片
- Fluorescein PEG
- 其他聚乙二醇试剂
- Phosphate PEG
- 蛋白质生物结合物
- NPC PEG
- Thiol PEG
Description:
DBCO(dibenzocycolctyne)PEGSilanederivativescangoClickchemistryreactionwithoutaneedofanymetalcatalysts.Thestrain-promoted1,3-dipolarcycloadditionofcyclooctynesandazides,alsotermedastheCu-freeclickreaction,isabioorthogonalreactionthatenablestheconjugationoftwomoleculesinaqueoussolution.DBCOPEGderivativespossessfastkineticsandstABIlityinaqueousbuffer.DBCOreagentscanbeusedtolabelazide-modifiedbiomoleculesspontaneouswithouttheneedfortoxicCucatalysts.PEGylationcanincreasesolubilityandstabilityandreduceimmunogenicityofpeptidesandproteins.Itcanalsosuppressthenon-specificbindingofchargedmoleculestothemodifiedsurfaces.
PhysicalProperties:
- Whitesolidorviscousliquiddependsonmoleculeweight;
- Solubleinregularaqeoussolutionaswellasmostorganicsolvents;
Copper-freeClickreactionprocedures:
- Preparetheazide-containingreagentsinreactionbuffer.
- AddDBCOreagentstoazidecontainingreagents.Add1molequivalentoflimitingreagentto1.5~3.0molequivalentsofabundancereagent.
- Incubatethereactionatroomtemperaturefor2~4hoursor2~12hoursat40C.
- Purifyconjugateseitherbydialysisorsize-exclusionchromatograph.
StorageConditions:
- Storeat-200C,dessicate.Protectfromlight.Avoidfrequentthawandfreeze.