Cell line Informatrion:
Designations: BALB/3T3 clone A31Depositors: S AaronsonGrowth Properties: adherentOrganism: Mus musculusMorphology: fibroblastSource: embryoStrain: BALB/cCell Type: fibroblastIsolation date: 1968Applications: transfection hostVirus Susceptibility: Human poliovirus 1, Herpes simplex virus, Vesicular stomatitis virusTumorigenic: NoAge: embryo; 14 to 17 day gestationThe BALB/3T3 clone A31 is one of several cell lines developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos. The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus SV40 and murine sarcoma virus.
The Transfection Reagent:
BALB/3T3 clone A31 Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting BALB/3T3 clone A31 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for BALB/3T3 clone A31 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into BALB/3T3 clone A31 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
