Methionineaminopeptidase1,METAP,alsonamedMAP1,MetAP1,PeptidaseM1,isamemberoftheM24familyofmetalloproteases.METAPsplayacentralroleforproteinmaturationastheyremovetheinitiatorMetresiduefromnewlysynthesizedproteinsandareessentialforcellgrowth.ThreeMETAPgenes(METAP1,METAP2andMAP1D)havebeenidentifiedinthehumangenome.METAP1andMETAP2havedifferentsubstratespecificityduetothedifferencesinbothsizeandshapeoftheactivesites.METAP1playsanimportantroleinG(2)/Mphaseregulationofthecellcycleandmayserveasapromisingtargetforthediscoveryanddevelopmentofnewanticanceragents.Fumagillin,anaturalcompound,covalentlymodifiesanactiveHisresidueofMETAP2andpreventsthevascularizationandmetasisoftumors.
Source | E.coli |
Names | MethionineAminopeptidase,MAP,PeptidaseM,map |
Accession# | P0AE18 |
Formulation | Suppliedasa0.2μMfilteredsolutionof20mMTris-HCl,100mMNaCl,50%Glycerol,pH8.0 |
Shipping | Theproductisshippedondryice/icepacks. |
Storage | Storeat<-20°C,stablefor6monthsafterreceipt. Pleaseminimizefreeze-thawcycles. |
Purity | Greaterthan95%asdeterminedbySEC-HPLCandreducingSDS-PAGE. |
Endotoxin | Lessthan0.1ng/μg(1IEU/μg). |
AminoAcidSequence | MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRICNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIKDGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAEGFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMKDGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDELEHHHHHH |