LonProtease,isamemberoftheLonproteasefamily.Theyarefoundinarchaea,bacteriaandeukaryotes.LonproteaseisATP-dependentserineproteasethatmediatestheselectivedegradationofmutantandabnormalproteinsaswellascertainshort-livedregulatoryproteins,includingsomeantitoxins.ItrequiredforcellularhomeostasisandforsurvivalfromDNAdamageanddevelopmentalchangesinducedbystress.Itdegradespolypeptidesprocessivelytoyieldsmallpeptidefragmentsthatare5to10aminoacidslongandbindstoDNAinadouble-stranded,site-specificmanner.EndogenoussubstratesincludetheregulatoryproteinsRcsAandSulA,thetranscriptionalactivatorSoxS,andUmuD.Itsoverproductionspecificallyinhibitstranslationthroughatleasttwodifferentpathways,oneofthembeingtheYoeB-YefMtoxin-antitoxinsystem.
Source | E.coli |
Names | LonProtease,ATP-DependentProteaseLa,lon,capR,deg,lopA,muc |
Accession# | P0A9M0 |
Formulation | Suppliedasa0.2μMfilteredsolutionof50mMTris-HCl,100mMKCl,10%Glycerol,pH7.5 |
Shipping | Theproductisshippedondryice/icepacks. |
Storage | Storeat<-20°C,stablefor6monthsafterreceipt. Pleaseminimizefreeze-thawcycles. |
Purity | Greaterthan95%asdeterminedbySEC-HPLCandreducingSDS-PAGE. |
Endotoxin | Lessthan0.1ng/µg(1IEU/µg). |
AminoAcidSequence | MGNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAMDHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISALSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSIDDPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVKKQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQKLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRILEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIRGHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNVAFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLPKQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLKHIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKLTYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGIAMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFENKRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAKLEHHHHHH |