Tryptophansynthaseisamultienzymeα2β2complexcomposedoftwoproteinsubunit.TryptophansynthasecatalyzesthelasttwostepsinthesynthesisofL-tryptophan(L-Trp).Theα-subunitcatalyzescleavageof3-indole-d-glycerol3′-phosphate(IGP)togiveindoleandD-glyceraldehyde3′-phosphate(G3P).Indoleisthentransferredthrougha25-Åhydrophobictunneltotheβ-subunit.Theβ2subunitcontainspyridoxal5"-phosphateandcatalyzesseveralpyridoxal5"-phosphate-dependentreactions,including/3-eliminationreactions6andathiol-dependenttransaminationreaction.ThisenzymeiscommonlyfoundinEubacteria,Archaebacteria,Protista,Fungi,andPlantae,butisabsentfromAnimalia.Ashumansdonothavetryptophansynthase,thisenzymehasbeenexploredasapotentialdrugtarget.
Source | E.coli |
Description | RecombinantE.coliTryptophansynthaseisproducedbyourE.coliexpressionsystemandthetargetgeneencodingMet1-Ser268&Thr2-Ile397isexpressedwitha6HistagattheN-terminus. |
| Names | Tryptophansynthetase,Tryptophansynthase |
Accession# | P0A877&P0A879 |
Formulation | Lyophilizedfroma0.2μmfilteredsolutionofPBS,pH7.4. |
Shipping | Theproductisshippedatambienttemperature. |
Reconstitution | Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting. |
Storage | Lyophilizedproteinshouldbestoredat<-20°C,thoughstableatroomtemperaturefor3weeks. |
Purity | Greaterthan95%asdeterminedbyreducingSDS-PAGE. |
Endotoxin | Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest. |
AminoAcidSequence | MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS&MHHHHHHTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQFNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILEREGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALEAFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILKARGEI | Everest Biotech自2000年以来，我们一直是抗肽和抗原亲和纯化山羊多克隆抗体的专家。我们的抗体具有100％的满意保证-它们可在您的实验室或您的退款中发挥作用。 LifeSpan BioSciences，Inc.收购了Everest Biotech。 在此处查找使用Everest抗体的最新出版物盖玻片 用于悬浮细胞免疫荧光
