Tryptophansynthaseisanenzymethatcatalyzesthefinaltwostepsinthebiosynthesisoftryptophan.ItiscommonlyfoundinEubacteria,Archaebacteria,Protista,Fungi,andPlantae,butisabsentfromanimalssuchashumans.Tryptophansynthasetypicallyexistsasanα-ββ-αcomplex.ThealphasubunitisresponsIBLeforthealdolcleavageofindoleglycerolphosphatetoindoleandglyceraldehyde3-phosphate:L-serine+1-C-(indol-3-yl)glycerol3-phosphate=L-tryptophan+D-glyceraldehyde3-phosphate+H2O.Thebetasubunitscatalyzetheirreversiblecondensationofindoleandserinetoformtryptophaninapyridoxalphosphate(PLP)dependentreaction.Theirassemblyintoacomplexleadstostructuralchangesinbothsubunitsresultinginreciprocalactivation
Source | E.coli |
Description | RecombinantE.coliTryptophansynthasealphachainisproducedbyourE.coliexpressionsystemandthetargetgeneencodingMet1-Ser268isexpressed. |
Names | Tryptophansynthasealphachain,trpA |
Accession# | P0A877 |
Formulation | Lyophilizedfroma0.2μmfilteredsolutionofPBS,pH7.4. |
Shipping | Theproductisshippedatambienttemperature. |
Reconstitution | Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting. Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100μg/ml. DissolvethelyophilizedproteininddH2O. Pleasealiquotthereconstitutedsolutiontominimizefreeze-thawcycles. |
Storage | Lyophilizedproteinshouldbestoredat<-20°C,thoughstableatroomtemperaturefor3weeks. Reconstitutedproteinsolutioncanbestoredat4-7°Cfor2-7days. Aliquotsofreconstitutedsamplesarestableat<-20°Cfor3months. |
Purity | Greaterthan95%asdeterminedbyreducingSDS-PAGE. |
Endotoxin | Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest. |
AminoAcidSequence | MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS | Everest Biotech自2000年以来，我们一直是抗肽和抗原亲和纯化山羊多克隆抗体的专家。我们的抗体具有100％的满意保证-它们可在您的实验室或您的退款中发挥作用。 LifeSpan BioSciences，Inc.收购了Everest Biotech。 在此处查找使用Everest抗体的最新出版物盖玻片 用于悬浮细胞免疫荧光
