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商品描述
SerpinF1issecretedNeurotrophicproteinandbelongstotheserpinfamily.SerpinF1Highlyexpressedintheliver,gastricglandularmucosaandrenaltubules.Itisalsoexpressedinthebrain,heart,lungretinaandtestes.Itinducesextensiveneuronaldifferentiationinretinoblastomacells.Itispotentinhibitorofangiogenesis.AsitdoesnotundergotheS(stressed)toR(relaxed)conformationaltransitioncharacteristicofactiveserpins,exhibitsnoserineproteaseinhibitoryactivity.
| Source | E.coli |
| Names | Pigmentepithelium-derivedfactor,Pedf,Sdf3,Caspin,SerpinF1,Stromalcell-derivedfactor3. |
| Accession# | P97298 |
| Formulation | Lyophilizedfroma0.2μMfilteredsolutionofPBS,pH7.4 |
| Shipping | Theproductisshippedatambienttemperature. |
| Reconstitution | Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting. Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100μg/ml. Dissolvethelyophilizedproteinin3XPBS. Pleasealiquotthereconstitutedsolution |
| Storage | Lyophilizedproteinshouldbestoredat<-20°C,thoughstableatroomtemperaturefor3weeks. Reconstitutedproteinsolutioncanbestoredat4-7°Cfor2-7days. Aliquotsofreconstitutedsamplesarestableat<-20°Cfor5months. |
| Purity | Greaterthan95%asdeterminedbySEC-HPLCandreducingSDS-PAGE. |
| Endotoxin | Lessthan0.1ng/µg(1IEU/µg). |
| AminoAcidSequence | MDPFFKVPVNKLAAAVSNFGYDLYRLRSSASPTGNVLLSPLSVATALSALSLGAEHRTESVIHRALYYDLITNPDIHSTYKELLASVTAPEKNLKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRVDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWVTKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFIHDIDRELKTIQAVLTVPKLKLSFEGELTKSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGSSPSPGLQPVRLTFPLDYHLNQPFLFVLRDTDTGALLFIGRILDPSST |
