WesternblotAt0.5-1μg/mlwiththeappropriatesystemtodetectcalbindin-Dincellsandtissues.
Immunohistochemistry
At1-2μg/mltodetectcalbindin-Dinformalinfixedandparaffinembeddedtissues.
(Paraffin-embeddedSection)
Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.
Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.
1.Parmentier,M.;Passage,E.;Vassart,G.;Mattei,M.-G.:ThehumancalbindinD28k(CALB1)andcalretinin(CALB2)genesarelocatedat8q21.3-q22.1and16q22-q23,respectively,suggestingacommonduplicationwiththecarbonicanhydraseisozymeloci.Cytogenet.CellGenet.57:41-43,1991.
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