Immunohistochemistry | At2-4μg/mltodetectAFPinformalin-fixedandparaffinembeddedtissues. |
(Paraffin-embeddedSection) |
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Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.
Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.
1.D"Eustachio,P.;Ingram,R.S.;Tilghman,S.M.;Ruddle,F.H.:Murinealpha-fetoproteinandalbumin:twoevolutionarilylinkedproteinsencodedonthesamemousechromosome.Somat.CellGenet.7:289-294,1981.
2.Beattie,W.G.;Dugaiczyk,A.:Structureandevolutionofhumanalpha-fetoproteindeducedfrompartialsequenceofclonedCDNA.Gene20:415-422,1982.
3.Gabant,P.;Forrester,L.;Nichols,J.;VanReeth,T.;DeMees,C.;Pajack,B.;Watt,A.;Smitz,J.;Alexandre,H.;Szpirer,C.;Szpirer,J.:Alpha-fetoprotein,themajorfetalserumprotein,isnotessentialforembryonicdevelopmentbutisrequiredforfemalefertility.Proc.Nat.Acad.Sci.99:12865-12870,2002.
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