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蚂蚁淘在线 / 品牌中心 / Everest Biotech / Everest Biotech/Monoclonal Anti-α-Smooth Muscle Actin 100μg/vial/2005881001/100μg/vial
商品描述
Clone1A4Size100μg/vialFormlyophilizedIgtypemouseIgG2aSpecificityNocrossreactivitywithotherproteins.SpeciesHuman,mouse,rat.ImmunogenN-terminalsyntheticdecapeptideofα-smoothmuscleactin.ContentsMouseascitesfluid,1.2%sodiumacetate,2mgBSA,with0.01mgNaN3aspreservative.
WesternblotAt2μg/mlwiththeappropriatesystemtodetectα-SMAincellsandtissues.
Immunohistochemistry
At0.1-0.5μg/mltodetectα-SMAinformalinfixedandparaffinembeddedtissues.
(Paraffin-embeddedSection)
Immunohistochemistry
At0.1-0.5μg/mltodetectα-SMAinformalinoracetonefixedtissues.
(FrozenSection)
Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.
Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.
1.Kumar,M.S.;Hendrix,J.A.;Johnson,A.D.;Owens,G.K.:Smoothmusclealpha-actingenerequirestwoE-boxesforproperexpressioninvivoandisatargetofclassIbasichelix-loop-helixproteins.Circ.Res.92:840-847,2003.
2.Ueyama,H.;Bruns,G.;Kanda,N.:AssignmentofthevascularsmoothmuscleactingeneACTSAtohumanchromosome10.Jpn.J.Hum.Genet.35:145-150,1990.
