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蚂蚁淘在线 / 品牌中心 / Everest Biotech / Everest Biotech/Human Betacellulin/BTC ELISA Kit 1 plate/2903870015/1 plate
商品描述
ForquantitativedetectionofhumanBetacellulinincellculturesupernates,serumandplasma(heparin,EDTA).
TypicalDataObtainedfromHumanBetacellulin
(TMBreactionincubateat37°Cfor25min)
Concentration(pg/ml) | 0.0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.073 | 0.086 | 0.112 | 0.179 | 0.285 | 0.540 | 0.961 | 1.650 |
TypicalHumanBetacellulinELISAKitStandardCurve
ThisstandardcurvewasgeneratedatEtonfordemonstrationpurposeonly.Astandardcurvemustberunwitheachassay.
Range15.6pg/ml-1000pg/ml
Sensitivity<10pg/ml
SpecificityNaturalandrecombinanthumanBetacellulin
Cross-reactivityNodetectablecross-reactivitywithotherrelevantproteins
Storage
Storeat4°Cfor6months,at-20°Cfor12months.Avoidmultiplefreeze-thawcycles(Shippedwithwetice.)
Precision
Intra-AssayPrecision(Precisionwithinanassay)Threesamplesofknownconcentrationweretestedononeplatetoassessintra-assayprecision.
Inter-AssayPrecision(Precisionbetweenassays)Threesamplesofknownconcentrationweretestedinseparateassaystoassessinter-assayprecision.
Intra-AssayPrecision | Inter-AssayPrecision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 46 | 119 | 725 | 47 | 124 | 736 |
Standarddeviation | 2.35 | 6.31 | 45.68 | 2.73 | 7.69 | 58.14 |
CV(%) | 5.1 | 5.3 | 6.3 | 5.8 | 6.2 | 7.9 |
Principle
Eton’shumanBetacellulinELISAKitwasbasedonstandardsandwichenzyme-linkedimmune-sorbentassaytechnology.AmonoclonalantibodyfrommousespecificforBetacellulinhasbeenprecoatedonto96-wellplates.Standards(E.coli,D32-Y111)andtestsamplesareaddedtothewells,abiotinylateddetectionpolyclonalantibodyfromgoatspecificforBetacellulinisaddedsubsequentlyandthenfollowedbywashingwithPBSorTBSbuffer.Avidin-Biotin-PeroxidaseComplexwasaddedandunboundconjugateswerewashedawaywithPBSorTBSbuffer.HRPsubstrateTMBwasusedtovisualizeHRPenzymaticreaction.TMBwascatalyzedbyHRPtoproduceabluecolorproductthatchangedintoyellowafteraddingacidicstopsolution.ThedensityofyellowisproportionaltothehumanBetacellulinamountofsamplecapturedinplate.
KitComponents
Description | Quantity |
96-wellplateprecoatedwithanti-humanBetacellulinantibody | 1 |
LyophilizedrecombinanthumanBetacellulinstandard | 10ng/tube×2 |
Biotinylatedanti-humanBetacellulinantibody | 130μl(dilution1:100) |
Avidin-Biotin-PeroxidaseComplex(ABC) | 130μl(dilution1:100) |
Samplediluentbuffer | 30ml |
Antibodydiluentbuffer | 12ml |
ABCdiluentbuffer | 12ml |
TMBcolordevelopingagent |
