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Everest Biotech/BlueFast Protein Staining Solution 100 ml/1900011002/100 ml

价格
¥343.20
货号:1900011002
浏览量:127
品牌:Everest Biotech
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商品描述

Components

SKU#
1900011002
1900010013
Solution
100ml
10x100ml

Materialneededbutnotsupplied

1)Microwaveoven.
2)Microwavableplasticbox.

FeaturesandApplications
Sensitivity:Detectionofproteinaslowas25ng(BSA)canbeachievedin10min.Sensitivitycanbeextendedto10ngforsomeproteins(suchasBSA)withadditionalstainingtreatmentandwashingsteps.
Applications:DetectionofproteinsinnativeandSDS-PAGEgels.

Storage

Roomtemperatureinalightproofbottle.

Warning:Theinstructionsdescribedbelowinvolvehandingof“hot”liquid.Followthedirectionswithcautiontopreventburn.

1)Placeastandardminigelinamicrowavableplasticboxwith250mlofdeionizedwater(orsufficientamount)tocoverthegel.Looselycovertheboxandmicrowavethegelfor3min.(highsetting).

2)Discardthewater.Repeatstep1.

3)Optionalstep:Formaximumdetectionsensitivity,repeatstep1twomoretimes(toatotalof4waterwashings).

4)Submergethegelin50ml(orsufficientamount)ofBlueFastsolution.Looselycovertheboxandmicrowavethegelfor2min.(highsetting).Formaximumdetection,submergethegelwith100mlofBlueFastsolution,andmicrowavethegelfor4min.

5)Discardstain.Add250mlofdeionizedwater.Looselycovertheboxandmicrowavethegelfor2min.(highsetting).Discardwash.Add250mlofdeionizedwater.

6)Optionalstep:Formaximumdetectionsensitivity,repeatstep5.

7)Optionalstep:BlueFastleavesalight-bluebackground,whichdoesnotaffectthesensitivityofdetectiononthegel.Toremoveitcompletely,washthegel3times,30min.eachwithrocking,with250mlofdeionizedwater,orsimplyleavethegelinwaterovernight.

Q:WhatisBlueFast?

A:BlueFastisaproprietaryformulationofcoomassieblueG-250stainsolution.Itsuniqueformulaworksitsmagicwithheattospeeduptheprocessofproteinstainingwithhighdetectionsensitivity.

Q:WhatisthesensitivityofBlueFast?

A:BlueFastworksbestfordetectionofproteins15kDaandabove.It’sdetectionsensitivityvariesfromproteintoproteindependingontheircomposition.Ingeneral,BlueFastdetectsaslowas25ngofBSAinnativeandSDS-PAGEwithin10minutesusingthestandardprocedure.ItispossIBLetoincreasethesensitivityofBlueFastdetectiontoaslowas10ngofBSA.Toachievethis,repeatthewatertreatmentsteptwomoretimes(toatotalof4treatments),increasetheBlueFaststainingstepfrom2to4minutes,andrepeatthepoststainingwashwithheatingonemoretime.

Q:HowdoesthedetectionsensitivityofBlueFastcomparedtotrADItionalcoomassiebluestainingmethodandsilverstainingmethod?

A:BlueFastisbasedoncolloidalcoomassiebluestainingtechnologysoitismoresensitivethanthetraditionalcoomassiebluestainingmethod.SilverstainingprovideshigherdetectionsensitivityforsomeproteinsthanBlueFast.However,silverstainingismoredifficulttoworkwithanditshighdetectionsensitivityisnotuniversallyapplicabletoallproteins.

Q:Howdoesthewatertreatmentwork?

A:BoilingofthegelinwaterpriortotheBlueFaststepremovessubstancesthatcaninhibittheinteractionbetweenBlueFastäandproteinsinthegel.

Q:Whatistheeffectofboilingonpolyacrylamidegel?

A:Boilingdoesnotdestroythepolyacrylamidegel.Theonlybeneficialeffectofboilingonthepolyacrylamidegelisitfixesthegelsoitwillnotexpandinwater.

Q:WhyproteinsdetectedbyBlueFastshowalightershadeofbluecomparedtothecolorseenwhenusingthetraditionalcoomassiebluestainingmethod?

A:TheuniqueformulationofBlueFastäyieldsalighterblue-coloredstainedproteinthanthatproducedbythetraditionalcoomassiebluestainingmethod.Thisdoesnotdecreasethesensitivityofproteindetection.

Q:CanBlueFastbereused?

A:Yes,butwedon’trecommenditunlessoneisworkingwithabundantamountofproteins.Detectionsensitivitydecreases50%whenBlueFastisusedthesecondtime.Therefore,unlessthereisahighconcentrationofproteininthegel,wedonotrecommendusingBlueFastasecondtime.

Q:CanproteinsbetransferredtoamembranefromagelaftertheyhavebeenstainedbyBlueFast?

A:Noformostproteins.OnceproteinsarestainedbyBlueFast,theycannotbetransferred.ItismisleadingtoseebluebandsonamembraneifoneattemptstotransferBlueFaststainedproteinsfromgel.Thetransferredbluebandsarecoomassiebluedyes.SinceproteindetectionbyBlueFastissorapid,werecommendrunningtwoidenticalgelssimultaneously.Leaveonegelintheelectrophoresisapparatus,stainthesecondgelwithBlueFastandobtaintheresultswithin10minutes.Basedontheresultsofthestainedgel,theuserthencandecidewhethertotransfertheunstainedgeltoamembrane.

Q:CanIremovethelight-bluebackgroundproducedbyBlueFastcompletely?

A:BlueFastleavesalight-bluebackground,whichdoesnotaffectthesensitivityofdetectiononthegel.Tocompletelyremovethelight-bluebackground,onecanrepeatthepoststainingwashstepwithheatingseveraltimes,leavethegelin500mlofwaterandchangethewaterhourly,orsimplyleavethegelinwaterovernight.

Q:CanItreattheBlueFaststainedgelwithgeldryingreagentscontainingglycerolandethanol?

A:Yes.
NOTE:Werecommendwashingthegelstwice(10minuteseach)withwaterandthenprocessingthegelwithgeldryingreagents.

Q:CanIsequenceproteinsstainedbyBlueFastusingmassspec?

A:Yes.Excisetheproteinofinterestedfromthestainedgel.Washtheexcisedgel3timeswithwater(onehoureach)orleaveitinwaterovernight.Thenproceedtothesequencingprocedure.


MATERIALSAFETYDATASHEET
TRADENAME:BlueFastProteinStainingSolution
DATEOFISSUE:February8,2017
SKU#:1900011002(100ml),1900010013(1000ml)

TRADENAME:BlueFastProteinStainingSolution
Manufacturer:EtonBioscienceInc.,5820OberlinDrive,Suite108,SanDiego,CA92121
InformationTelephone:800.758.1630
EmergencyTelephone:800.758.1630

SECTIONII:COMPOSITION/INFORMATIONONINGREDIENTS

CHEMICALCHARACTERIZATION:
CAS#
TritonX-100
9002-93-1
BrilliantBlueG250
6104-58-1
HydrochloricAcid
7467-01-0
Ethanol
64-17-5
HAZARDOUSCOMPONENTS:Theproductcontainsnosubstanceswhichattheirgivenconcentration,areconsideredtobehazardoustohealth.

SECTIONIII:HAZARDIDENTIFICATION

Irritatingtoeyesandskin.Maycausesensitizationbyskincontact.

SECTIONIV:FIRSTAIDMEASURES

GeneralInformation:Removeandwashcontaminatedclothingpromptly.
INCASEOFINHALATION:Supplyfreshairandthenconsultadoctor.
INCASEOFEYECONTACT:Immediatelyflusheyeswithcopiousamountsofwaterfor15minutes.
INCASEOFSKINCONTACT:Immediatelywashskinwithsoapandcopiousamountsofwaterandconsultadoctor.Removecontaminatedclothingandshoes.Washclothingbeforereuse.Cleanshoesthoroughlybeforereuse.
IFSWALLOWED:Rinsemouthwithcopiousamountsofwater.Donotinducevomiting.Nevergiveanythingbymouthtoaunconsciousperson.Thenconsultadoctor.

SECTIONV:FIREFIGHTINGMEASURES

EXTINGUISHINGAGENTS:
Suitable:UseDrychemical,waterspray,carbondioxideorfoam.

SECTIONVI:ACCIDENTALRELEASEMEASURES

PRECAUTIONARYMEASURES:Nospecialmeasuresrequired.
CLEAN-UPPROCEDURES:Nospecialmeasuresrequired.

SECTIONVII:HANDLINGANDSTORAGE

HANDLING:Avoidinhalationofvapourormist.Keepawayfrosourcesofignition.Nosmoking.
STORAGE:Keepcontainertightlyclosedinadryandwell-ventilatedplace.

SECTIONVIII:EXPOSURECONTROLS/PERSONALPROTECTION

Theroutineprecautionarymeasuresforhandlingchemicalsshouldbefollowedsuchaswearingchemicalsafetygoggles,rubbergloves,rubberboots,NIOSH/MSHA-Approvedrespiratorwhenhandlingchemicalsandwashthoroughlyafterhandling.Handlechemicalswithcareandavoidcontactwitheyes,skin,oronclothing.Usesafetyshowerandeyebathasfirstaidmeasuresifexposedtochemicals.

SECTIONIX:PHYSICALANDCHEMICALPROPERTIES

PHYSICALSTATE/FORM:Liquid
TRADENAME:BluefastProteinStainingSolution
COLOR:Blue
ODOR:None
pHFACTOR:(20°C)2.5-3.5
VISCOSITY:(20°C)N/A
MELTINGPOINT:N/A
BOILINGPOINT:N/A
IGNITIONTEMPERATURE:N/A
FLASHPOINT:N/A
EXPLOSIONLEVEL:LOWER:N/A
UPPER:N/A
VAPORPRESSURE:(20°C)N/A
SPECIFICGRAVITY:(20°C)N/A
SOLUBILITYINWATER:(20°C)Soluble

SECTIONX:STABILITYANDREACTIVITY

SUBSTANCESTOBEAVOIDED:Oxidizingagents.
DANGEROUSREACTIONS:Nodangerousreactionsknown.
HAZARDOUS,COMBUSTION,ORDECOMPOSITIONPRODUCTS:
Undernormalconditionsofstorageandhandling,hazardous,combustionordecompositionproductsshouldnotbeproduced.

SECTIONXI:TOXICOLOGICALINFORMATION

TOXICITYDATA:N/A
ACUTEEFFECTSINHALATION:N/A.
EYECONTACT:Maycauseeyeirritation.
SKINCONTACT:Maycauseskinirritation.
INGESTION:Maybeharmfulifswallowed.
PROLONGEDEXPOSURE:N/A
CHRONICEFFECTS:N/A
RTECSnumber:N/A
ADDITIONALINFORMATION:
Theproductshouldbehandledwithroutineprecautionarymeasuresforhandlingchemicals.Additionalharmfulpropertiescannotberuledout.

SECTIONXII:ECOLOGICALINFORMATION

Noinformationavailable.

SECTIONXIII:DISPOSALCONSIDERATIONS

Chemicalwastegeneratorsmustdeterminewhetheradiscardedchemicalisclassifiedasahazardouswaste.USEPAguidelinesfortheclassificationdeterminationarelistedin40CFRParts261.3.Additionally,wastegeneratorsmustconsultstateandlocalhazardouswasteregulationstoensurecompleteandaccurateclassification.

SECTIONXIV:TRANSPORTINFORMATION

DOTREGULATIONS:
UNNumber:1170Class:3Packinggroup:III
ProperShippingName:EthanolSolutions

SECTIONXV:REGULATORYINFORMATION

Noinformationavailable.

SECTIONXVI:OTHERINFORMATION


T
heinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation,andweassumenoliabilityresultingfromitsuse.Usersshouldmaketheirowninvestigationstodeterminethesuitabilityoftheinformationfortheirparticularpurposes.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.

Everest Biotech自2000年以来,我们一直是抗肽和抗原亲和纯化山羊多克隆抗体的专家。我们的抗体具有100%的满意保证-它们可在您的实验室或您的退款中发挥作用。 LifeSpan BioSciences,Inc.收购了Everest Biotech。 在此处查找使用Everest抗体的最新出版物盖玻片  用于悬浮细胞免疫荧光 
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