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蚂蚁淘在线 / 品牌中心 / Everest Biotech / Everest Biotech/BlueFast Protein Staining Solution 100 ml/1900011002/100 ml
商品描述
Components
SKU# | 1900011002 | 1900010013 |
Solution | 100ml | 10x100ml |
Materialneededbutnotsupplied
1)Microwaveoven.
2)Microwavableplasticbox.
FeaturesandApplications
Sensitivity:Detectionofproteinaslowas25ng(BSA)canbeachievedin10min.Sensitivitycanbeextendedto10ngforsomeproteins(suchasBSA)withadditionalstainingtreatmentandwashingsteps.
Applications:DetectionofproteinsinnativeandSDS-PAGEgels.
Storage
Roomtemperatureinalightproofbottle.
Warning:Theinstructionsdescribedbelowinvolvehandingof“hot”liquid.Followthedirectionswithcautiontopreventburn.
1)Placeastandardminigelinamicrowavableplasticboxwith250mlofdeionizedwater(orsufficientamount)tocoverthegel.Looselycovertheboxandmicrowavethegelfor3min.(highsetting).
2)Discardthewater.Repeatstep1.
3)Optionalstep:Formaximumdetectionsensitivity,repeatstep1twomoretimes(toatotalof4waterwashings).
4)Submergethegelin50ml(orsufficientamount)ofBlueFastsolution.Looselycovertheboxandmicrowavethegelfor2min.(highsetting).Formaximumdetection,submergethegelwith100mlofBlueFastsolution,andmicrowavethegelfor4min.
5)Discardstain.Add250mlofdeionizedwater.Looselycovertheboxandmicrowavethegelfor2min.(highsetting).Discardwash.Add250mlofdeionizedwater.
6)Optionalstep:Formaximumdetectionsensitivity,repeatstep5.
7)Optionalstep:BlueFastleavesalight-bluebackground,whichdoesnotaffectthesensitivityofdetectiononthegel.Toremoveitcompletely,washthegel3times,30min.eachwithrocking,with250mlofdeionizedwater,orsimplyleavethegelinwaterovernight.
Q:WhatisBlueFast?
A:BlueFastisaproprietaryformulationofcoomassieblueG-250stainsolution.Itsuniqueformulaworksitsmagicwithheattospeeduptheprocessofproteinstainingwithhighdetectionsensitivity.
Q:WhatisthesensitivityofBlueFast?
A:BlueFastworksbestfordetectionofproteins15kDaandabove.It’sdetectionsensitivityvariesfromproteintoproteindependingontheircomposition.Ingeneral,BlueFastdetectsaslowas25ngofBSAinnativeandSDS-PAGEwithin10minutesusingthestandardprocedure.ItispossIBLetoincreasethesensitivityofBlueFastdetectiontoaslowas10ngofBSA.Toachievethis,repeatthewatertreatmentsteptwomoretimes(toatotalof4treatments),increasetheBlueFaststainingstepfrom2to4minutes,andrepeatthepoststainingwashwithheatingonemoretime.
Q:HowdoesthedetectionsensitivityofBlueFastcomparedtotrADItionalcoomassiebluestainingmethodandsilverstainingmethod?
A:BlueFastisbasedoncolloidalcoomassiebluestainingtechnologysoitismoresensitivethanthetraditionalcoomassiebluestainingmethod.SilverstainingprovideshigherdetectionsensitivityforsomeproteinsthanBlueFast.However,silverstainingismoredifficulttoworkwithanditshighdetectionsensitivityisnotuniversallyapplicabletoallproteins.
Q:Howdoesthewatertreatmentwork?
A:BoilingofthegelinwaterpriortotheBlueFaststepremovessubstancesthatcaninhibittheinteractionbetweenBlueFastäandproteinsinthegel.
Q:Whatistheeffectofboilingonpolyacrylamidegel?
A:Boilingdoesnotdestroythepolyacrylamidegel.Theonlybeneficialeffectofboilingonthepolyacrylamidegelisitfixesthegelsoitwillnotexpandinwater.
Q:WhyproteinsdetectedbyBlueFastshowalightershadeofbluecomparedtothecolorseenwhenusingthetraditionalcoomassiebluestainingmethod?
A:TheuniqueformulationofBlueFastäyieldsalighterblue-coloredstainedproteinthanthatproducedbythetraditionalcoomassiebluestainingmethod.Thisdoesnotdecreasethesensitivityofproteindetection.
Q:CanBlueFastbereused?
A:Yes,butwedon’trecommenditunlessoneisworkingwithabundantamountofproteins.Detectionsensitivitydecreases50%whenBlueFastisusedthesecondtime.Therefore,unlessthereisahighconcentrationofproteininthegel,wedonotrecommendusingBlueFastasecondtime.
Q:CanproteinsbetransferredtoamembranefromagelaftertheyhavebeenstainedbyBlueFast?
A:Noformostproteins.OnceproteinsarestainedbyBlueFast,theycannotbetransferred.ItismisleadingtoseebluebandsonamembraneifoneattemptstotransferBlueFaststainedproteinsfromgel.Thetransferredbluebandsarecoomassiebluedyes.SinceproteindetectionbyBlueFastissorapid,werecommendrunningtwoidenticalgelssimultaneously.Leaveonegelintheelectrophoresisapparatus,stainthesecondgelwithBlueFastandobtaintheresultswithin10minutes.Basedontheresultsofthestainedgel,theuserthencandecidewhethertotransfertheunstainedgeltoamembrane.
Q:CanIremovethelight-bluebackgroundproducedbyBlueFastcompletely?
A:BlueFastleavesalight-bluebackground,whichdoesnotaffectthesensitivityofdetectiononthegel.Tocompletelyremovethelight-bluebackground,onecanrepeatthepoststainingwashstepwithheatingseveraltimes,leavethegelin500mlofwaterandchangethewaterhourly,orsimplyleavethegelinwaterovernight.
Q:CanItreattheBlueFaststainedgelwithgeldryingreagentscontainingglycerolandethanol?
A:Yes.
NOTE:Werecommendwashingthegelstwice(10minuteseach)withwaterandthenprocessingthegelwithgeldryingreagents.
Q:CanIsequenceproteinsstainedbyBlueFastusingmassspec?
A:Yes.Excisetheproteinofinterestedfromthestainedgel.Washtheexcisedgel3timeswithwater(onehoureach)orleaveitinwaterovernight.Thenproceedtothesequencingprocedure.
MATERIALSAFETYDATASHEET
TRADENAME:BlueFastProteinStainingSolution
DATEOFISSUE:February8,2017
SKU#:1900011002(100ml),1900010013(1000ml)
TRADENAME:BlueFastProteinStainingSolution
Manufacturer:EtonBioscienceInc.,5820OberlinDrive,Suite108,SanDiego,CA92121
InformationTelephone:800.758.1630
EmergencyTelephone:800.758.1630
SECTIONII:COMPOSITION/INFORMATIONONINGREDIENTS
CHEMICALCHARACTERIZATION:
CAS# | |
TritonX-100 | 9002-93-1 |
BrilliantBlueG250 | 6104-58-1 |
HydrochloricAcid | 7467-01-0 |
Ethanol | 64-17-5 |
HAZARDOUSCOMPONENTS:Theproductcontainsnosubstanceswhichattheirgivenconcentration,areconsideredtobehazardoustohealth.
SECTIONIII:HAZARDIDENTIFICATION
Irritatingtoeyesandskin.Maycausesensitizationbyskincontact.
SECTIONIV:FIRSTAIDMEASURES
GeneralInformation:Removeandwashcontaminatedclothingpromptly.
INCASEOFINHALATION:Supplyfreshairandthenconsultadoctor.
INCASEOFEYECONTACT:Immediatelyflusheyeswithcopiousamountsofwaterfor15minutes.
INCASEOFSKINCONTACT:Immediatelywashskinwithsoapandcopiousamountsofwaterandconsultadoctor.Removecontaminatedclothingandshoes.Washclothingbeforereuse.Cleanshoesthoroughlybeforereuse.
IFSWALLOWED:Rinsemouthwithcopiousamountsofwater.Donotinducevomiting.Nevergiveanythingbymouthtoaunconsciousperson.Thenconsultadoctor.
SECTIONV:FIREFIGHTINGMEASURES
EXTINGUISHINGAGENTS:
Suitable:UseDrychemical,waterspray,carbondioxideorfoam.
SECTIONVI:ACCIDENTALRELEASEMEASURES
PRECAUTIONARYMEASURES:Nospecialmeasuresrequired.
CLEAN-UPPROCEDURES:Nospecialmeasuresrequired.
SECTIONVII:HANDLINGANDSTORAGE
HANDLING:Avoidinhalationofvapourormist.Keepawayfrosourcesofignition.Nosmoking.
STORAGE:Keepcontainertightlyclosedinadryandwell-ventilatedplace.
SECTIONVIII:EXPOSURECONTROLS/PERSONALPROTECTION
Theroutineprecautionarymeasuresforhandlingchemicalsshouldbefollowedsuchaswearingchemicalsafetygoggles,rubbergloves,rubberboots,NIOSH/MSHA-Approvedrespiratorwhenhandlingchemicalsandwashthoroughlyafterhandling.Handlechemicalswithcareandavoidcontactwitheyes,skin,oronclothing.Usesafetyshowerandeyebathasfirstaidmeasuresifexposedtochemicals.
SECTIONIX:PHYSICALANDCHEMICALPROPERTIES
PHYSICALSTATE/FORM:Liquid
TRADENAME:BluefastProteinStainingSolution
COLOR:Blue
ODOR:None
pHFACTOR:(20°C)2.5-3.5
VISCOSITY:(20°C)N/A
MELTINGPOINT:N/A
BOILINGPOINT:N/A
IGNITIONTEMPERATURE:N/A
FLASHPOINT:N/A
EXPLOSIONLEVEL:LOWER:N/A
UPPER:N/A
VAPORPRESSURE:(20°C)N/A
SPECIFICGRAVITY:(20°C)N/A
SOLUBILITYINWATER:(20°C)Soluble
SECTIONX:STABILITYANDREACTIVITY
SUBSTANCESTOBEAVOIDED:Oxidizingagents.
DANGEROUSREACTIONS:Nodangerousreactionsknown.
HAZARDOUS,COMBUSTION,ORDECOMPOSITIONPRODUCTS:
Undernormalconditionsofstorageandhandling,hazardous,combustionordecompositionproductsshouldnotbeproduced.
SECTIONXI:TOXICOLOGICALINFORMATION
TOXICITYDATA:N/A
ACUTEEFFECTSINHALATION:N/A.
EYECONTACT:Maycauseeyeirritation.
SKINCONTACT:Maycauseskinirritation.
INGESTION:Maybeharmfulifswallowed.
PROLONGEDEXPOSURE:N/A
CHRONICEFFECTS:N/A
RTECSnumber:N/A
ADDITIONALINFORMATION:
Theproductshouldbehandledwithroutineprecautionarymeasuresforhandlingchemicals.Additionalharmfulpropertiescannotberuledout.
SECTIONXII:ECOLOGICALINFORMATION
Noinformationavailable.
SECTIONXIII:DISPOSALCONSIDERATIONS
Chemicalwastegeneratorsmustdeterminewhetheradiscardedchemicalisclassifiedasahazardouswaste.USEPAguidelinesfortheclassificationdeterminationarelistedin40CFRParts261.3.Additionally,wastegeneratorsmustconsultstateandlocalhazardouswasteregulationstoensurecompleteandaccurateclassification.
SECTIONXIV:TRANSPORTINFORMATION
DOTREGULATIONS:
UNNumber:1170Class:3Packinggroup:III
ProperShippingName:EthanolSolutions
SECTIONXV:REGULATORYINFORMATION
Noinformationavailable.
SECTIONXVI:OTHERINFORMATION
Theinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation,andweassumenoliabilityresultingfromitsuse.Usersshouldmaketheirowninvestigationstodeterminethesuitabilityoftheinformationfortheirparticularpurposes.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.
Everest Biotech自2000年以来，我们一直是抗肽和抗原亲和纯化山羊多克隆抗体的专家。我们的抗体具有100％的满意保证-它们可在您的实验室或您的退款中发挥作用。 LifeSpan BioSciences，Inc.收购了Everest Biotech。 在此处查找使用Everest抗体的最新出版物盖玻片 用于悬浮细胞免疫荧光
