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蚂蚁淘在线 / 品牌中心 / Athens Research / Athens Research/Cambio - Excellence in Molecular Biology/2.0g/10-1015-20

Athens Research/Cambio - Excellence in Molecular Biology/2.0g/10-1015-20

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Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

Ac-dC-CE Phosphoramidite

Glen Research

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
10-1015-02	Ac-dC-CE Phosphoramidite	0.25g	£30.00£28.50Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-02M	Ac-dC-CE Phosphoramidite	0.25g	£30.00£28.50Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-05	Ac-dC-CE Phosphoramidite	0.5g	£30.00£28.50Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-05M	Ac-dC-CE Phosphoramidite	0.5g	£30.00£28.50Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-10	Ac-dC-CE Phosphoramidite	1.0g	£40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-10M	Ac-dC-CE Phosphoramidite	1.0g	£40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-1C	Ac-dC-CE Phosphoramidite	1.0g	£40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-1S	Ac-dC-CE Phosphoramidite	10.0g	£200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-20	Ac-dC-CE Phosphoramidite	2.0g	£80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-2C	Ac-dC-CE Phosphoramidite	2.0g	£80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-40	Ac-dC-CE Phosphoramidite	4.0g	£80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-50	Ac-dC-CE Phosphoramidite	5.0g	£100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-5S	Ac-dC-CE Phosphoramidite	5.0g	£100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-C2	Ac-dC-CE Phosphoramidite	0.25g	£30.00£28.50Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1015-C5	Ac-dC-CE Phosphoramidite	0.5g	£30.00£28.50Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order

Ac-dC-CE Phosphoramidite

Glen Research

Ac-dC-CE Phosphoramidite

Structure

Catalog Number: 10-1015-xx

Description: Ac-dC-CE Phosphoramidite

5"-Dimethoxytrityl-N-acetyl-2"-deoxyCytidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C₄₁H₅₀N₅O₈P	M.W.: 771.85	F.W.: 289.18

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Storage: Controlled room temperature or lower, dry

Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

STERLING CE PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Ac-dC-CE Phosphoramidite

Glen Research

MSDS

Glen Report 6.2: ULTRAFAST DNA SYNTHESIS - 10 MINUTE DEPROTECTION!

Glen Report 11.2: ALTERNATIVES TO EXPEDITE MONOMERS

Glen Report 7.1: ULTRAFAST CHEMISTRY

Glen Report 19.2: New Lines of Monomers - HT for High Throughput and LC for low cost RN

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Ac-dC-CE Phosphoramidite

Glen Research

Frequently Asked Technical Question

QUESTION: How does this work? What would happen if I used the AMA reagent with Bz protected dC?

RESPONSE:dC when proected as an amide linkage is susceptible to transamination or displacement of the amide with the deprotecting amine. Normally, the deprotecting amine is ammonia and dispacement leads to the same product as hydrolysis. However, if methylamine is used for the deprotection step with a benzoyl protected dC, approximately 10% of the product is from the displacement reaction to give N-methyl-dC residues, while the remaining 90% is the desired product formed by hydrolysis. A more labile protecting group on dC is isobutyryl which is also commercially available. This group is displaced by methylamine to about 5% - an improvement but still a mutation. Ac-dC, on the other hand, is hydrolyzed virtually instantaneously by the AMA reagent and none of the slower displacement reaction gets a chance to occur.

REFERENCE(S):M.P. Reddy, Beckman Instruments, personal communication.

QUESTION: Can the AMA reagent be made up and stored? for how long? in what kind of container?

RESPONSE:Yes. Store tightly sealed in a glass bottle in the refrigerator. The reagent does not decompose but routine opening will lose some of the volatile bases. Dispose properly after 4-6 weeks.

REFERENCE(S):Candy Lee, Dartmouth

QUESTION: What is the procedure for deprotecting with AMA?

RESPONSE:The AMA reagent is a 50:50 mixture of aqueous Ammonium

hydroxide and aqueous MethylAmine. With AMA the cleavage of the

oligonucleotide from the support is accomplished in 5 minutes at room

temperature. The deprotection step is carried out at 65°C for a

further 5 minutes. Deprotection can also be carried out at lower

temperatures as shown in Table 1. In all cases, no base modification

has been observed.

TABLE 1: DEPROTECTION WITH AMA

Time	Temperature
5 min	65°C
10 min	55°C
30 min	37°C
90 min	25°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Ac-dC-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.	PackSize	Grams/Pack	0.1M Dil.(mL)	LV40	LV200	40nm	0.2µm	1µm	10µm
Cat.No.	PackSize	Grams/Pack	0.1M Dil.(mL)	Approximate Number of Additions
10-1015-02	0.25grams	.25grams	3.24	94.67	56.8	35.5	25.82	18.93	4.73
10-1015-05	0.5grams	.5grams	6.48	202.67	121.6	76	55.27	40.53	10.13
10-1015-10	1.0gram	1grams	12.96	418.67	251.2	157	114.18	83.73	20.93
10-1015-20	2.0grams	2grams	25.91	850.33	510.2	318.88	231.91	170.07	42.52
10-1015-40	4.0grams	4grams	51.82	1714	1028.4	642.75	467.45	342.8	85.7
Expedite
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Molarity	50nm	0.2µm	1µm	15µm
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Approximate Number of Additions
10-1015-1C	1.0gram	1grams	19.34	.07	383.6	239.75	174.36	23.98
10-1015-2C	2.0grams	2grams	38.67	.07	770.2	481.38	350.09	48.14
10-1015-C2	0.25grams	.25grams	4.83	.07	93.4	58.38	42.45	5.84
10-1015-C5	0.5grams	.5grams	9.67	.07	190.2	118.88	86.45	11.89
Beckman
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Molarity	30nm	200nm	1000nm
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Approximate Number of Additions
10-1015-1B	1.0gram	1grams	19.34	.07	382	238.75	173.64
10-1015-B2	0.25grams	.25grams	4.83	.07	91.8	57.38	41.73
10-1015-B5	0.5grams	.5grams	9.67	.07	188.6	117.88	85.73

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.