Athens Research/Cambio - Excellence in Molecular Biology/5 pouches/MC-12-9193-5

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货号:MC-12-9193-5
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Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago"s SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility

  • Catalogue
  • Description
  • Notes
  • Applications & Benefits

Sodium dodecyl sulphate (SDS)

The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
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MC-09-2026-1000Sodium dodecyl sulphate (SDS) 0.5 g1000 tablets£218.00QuantityAdd to Order
MC-09-2026-50Sodium dodecyl sulphate (SDS) 0.5 g50 tablets£23.00QuantityAdd to Order
MC-12-9193-5Sodium dodecyl sulphate (SDS) 10 %, 1000 ml5 pouches£292.00QuantityAdd to Order
MC-12-9194-5Sodium dodecyl sulphate (SDS) 20 %, 1000 ml5 pouches£502.00QuantityAdd to Order

Sodium dodecyl sulphate (SDS)

The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain.

Medicago AB

Product description

The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain. As a result, the distance of migration through the gel in SDS-PAGE electrophoresis can be assumed to be directly related only to the size of the protein.

Features

  • Prepared from analytical grade reagent
  • Anionic detergent
  • Denaturing agent used in SDS-PAGE
  • Choice of tablets and powder in pouches
  • Dissolve and use in minutes

Medicago’s SDS reagent is supplied in pre-weighed tablets. One tablet weighs 0.5 g and can be dissolved in deionized water to the desired volume and concentration. SDS reagent powder mix in pouches give 1000 ml of 10% or 20% sodium dodecyl sulphate solution.

Directions for use

Empty one pouch or deposit one tablet of the SDS reagent in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 ml, stir until full dissolution and the solution is ready to use.

Deposit one tablet of the SDS reagent in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water and stir the solution for a few minutes until full dissolution. Adjust the volume up to required concentration and the solution is ready to use.

Shipping and storage

The SDS reagent is shipped at room temperature. Store the tablets and pouches in a dry place at room temperature. Shelf life is three years after production date.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Sodium dodecyl sulphate (SDS)

The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain.

Medicago AB

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed tablets or powder

Concentration: 10% or 20% solution

Weight: 0.5 g/tablet

Volume: 1000 ml

pH: 3.0 ± 0.05 at 25°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Sodium dodecyl sulphate (SDS)

The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain.

Medicago AB

Applications

  • Solubilizes and denatures proteins
  • SDS-PAGE electrophoresis
  • Western blots

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶 免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.