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蚂蚁淘在线 / 品牌中心 / Athens Research / Athens Research/Cambio - Excellence in Molecular Biology/1U/µl 1,000U/UG131K

Athens Research/Cambio - Excellence in Molecular Biology/1U/µl 1,000U/UG131K

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货号：UG131K
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商品描述
Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

  • Catalogue
  • Description
  • Protocols
  • References
  • Notes
  • Applications & Benefits

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
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UG131KUracil N-Glycosylase (UNG)1U/µl 1,000U£1,025.00QuantityAdd to Order

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Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Formally known as HK™-UNG Thermolabile Uracil N-Glycosylase and the previous numbers were HU5901K and HU59100.

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate. UNG is ideal for studying repair of abasic sites in double-stranded DNA.

Unit Definition: One unit of UNG catalyzes the release of 1 nmol of uracil from uracil-containing DNA in 1 hour at 37°C under standard assay conditions.

Note: Epicentre"s unit is 5- to 20-fold more active than a unit as defined by other suppliers. Therefore, Dilution Buffer is provided for applications requiring lower concentrations of enzyme.

Dilution and Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

Quality Control: UNG is free of detectable exo- and endonuclease and RNase activities.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG)

(catalogue number UG13100 or UG131K)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Note: The product name and catalog numbers have changed. The previous name was HK™-UNG Thermolabile Uracil N-Glycosylase and the previous numbers were HU5901K and HU59100.
  1. Hamberger, B., et al. (2011) Evolution of Diterpene Metabolism: Sitka Spruce CYP720B4 Catalyzes Multiple Oxidations in Resin Acid Biosynthesis of Conifer Defense against Insects. Plant Physiology 157, 1677-1695
  2. MacFarlane, A. J., et al. (2011) Nuclear Localization of de Novo Thymidylate Biosynthesis Pathway Is Required to Prevent Uracil Accumulation in DNA. J. Biol. Chem. 286, 44015-44022
  3. Jaijo, T., et al. (2010) Microarray-Based Mutation Analysis of 183 Spanish Families with Usher Syndrome. Invest. Ophthalmol. Vis. Sci. 51, 1311-1317
  4. Tat, S. K., et al. (2010) Variable Effects of 3 Different Chondroitin Sulfate Compounds on Human Osteoarthritic Cartilage/Chondrocytes: Relevance of Purity and Production Process. J Rheumatol 37, 656-664
  5. Petraitis, V., et al. (2009) Combination Therapy in Treatment of Experimental Pulmonary Aspergillosis: In Vitro and In Vivo Correlations of the Concentration- and Dose- Dependent Interactions between Anidulafungin and Voriconazole by Bliss Independence Drug Interaction Analysis. Antimicrob. Agents Chemother. 53, 2382-2391
  6. Tang, Y.-W. (2009) Duplex PCR Assay Simultaneously Detecting and Differentiating Bartonella quintana, B. henselae, and Coxiella burnetii in Surgical Heart Valve Specimens. J. Clin. Microbiol. 47, 2647-2650
  7. Francesconi, A., et al. (2008) Automated and Manual Methods of DNA Extraction for Aspergillus fumigatus and Rhizopus oryzae Analyzed by Quantitative Real-Time PCR. J. Clin. Microbiol. 46, 1978-1984
  8. Kasai, M., et al. (2008) Detection of a Molecular Biomarker for Zygomycetes by Quantitative PCR Assays of Plasma, Bronchoalveolar Lavage, and Lung Tissue in a Rabbit Model of Experimental Pulmonary Zygomycosis. J. Clin. Microbiol. 46, 3690-3702
  9. Francesconi, A., et al. (2006) Characterization and Comparison of Galactomannan Enzyme Immunoassay and Quantitative Real-Time PCR Assay for Detection of Aspergillus fumigatus in Bronchoalveolar Lavage Fluid from Experimental Invasive Pulmonary Aspergillosis. J. Clin. Microbiol. 44, 2475-2480
  10. Kasai, M., et al. (2006) Use of Quantitative Real-Time PCR To Study the Kinetics of Extracellular DNA Released from Candida albicans, with Implications for Diagnosis of Invasive Candidiasis. J. Clin. Microbiol. 44, 143-150
  11. Elfaitouri, A., et al. (2005) Quantitative PCR-Enhanced Immunoassay for Measurement of Enteroviral Immunoglobulin M Antibody and Diagnosis of Aseptic Meningitis. Clin. Diagn. Lab. Immunol. 12, 235-241
  12. Hadd, A. G., et al. (2004) Microsphere Bead Arrays and Sequence Validation of 5/7/9T Genotypes for Multiplex Screening of Cystic Fibrosis Polymorphisms. J. Mol. Diagn. 6, 348-355
  13. O"Sullivan, C. E., et al. (2003) Development and Validation of a Quantitative Real-Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis. J. Clin. Microbiol. 41, 5676-5682
  14. Sloan, L. M., et al. (2002) Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens. J. Clin. Microbiol. 40, 96-100
  15. Smalling, T. W., et al. (2002) Molecular Approaches To Detecting Herpes Simplex Virus and Enteroviruses in the Central Nervous System. J. Clin. Microbiol. 40, 2317-2322
  16. Schwab, K. J., et al. (2001) Development of a Reverse Transcription-PCR-DNA Enzyme Immunoassay for Detection of "Norwalk-Like" Viruses and Hepatitis A Virus in Stool and Shellfish. Appl. Envir. Microbiol. 67, 742-749
  17. Atamna, H., et al. (2000) A method for detecting abasic sites in living cells: Age-dependent changes in base excision repair. PNAS 97, 686-691
  18. Pinz, K. G. and Bogenhagen, D. F. (2000) Characterization of a Catalytically Slow AP Lyase Activity in DNA Polymerase gamma and Other Family A DNA Polymerases. J. Biol. Chem. 275, 12509-12514
  19. Saurin, J. C., et al. (2000) Detection of Ki-ras gene point mutations in bile specimens for the differential diagnosis of malignant and benign biliary strictures. Gut 47, 357-361
  20. Zazzi, M., et al. (1999) Clinical Evaluation of an In-House Reverse Transcription-Competitive PCR for Quantitation of Human Immunodeficiency Virus Type 1RNA in Plasma. J. Clin. Microbiol. 37, 333-338
  21. Bogenhagen, D. F. and Pinz, K. G. (1998) The Action of DNA Ligase at Abasic Sites in DNA. J. Biol. Chem. 273, 7888-7893
  22. Pinz, K. G. and Bogenhagen, D. F. (1998) Efficient Repair of Abasic Sites in DNA by Mitochondrial Enzymes. Mol. Cell. Biol. 18, 1257-1265

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Note: This product is accompanied by a limited non-exclusive license for the purchaser to use the purchased product for Base Excision Sequence Scanning (BESS-T&G™ Scanning) in life science research under U.S. Patent No. 6,048,696 and similar patents pending in the U.S. and other countries. No other license for any other use or field of use is either granted or implied by the sale of this product. Also see Section 6 for information on BESS-T&G™ Base Reader Kits.

Note: EPICENTRE"s unit is 5- to 20-fold more active than a unit as defined by other suppliers. Therefore, Dilution Buffer is provided for applications requiring lower concentrations of enzyme

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Uracil N-Glycosylase (UNG)

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • DNA repair studies

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶 免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.