Cell culture contamination control: Detection

AquaScreen® is an in vitro test system for the qualitative and/or quantitative detection of water pathogens like Legionella pneumophila and Legionella species.AquaScreen® combines a sample preparation module with PCR analysis. The system allows highly sensitive analysis of water samples.

The supplied primer set is specific for a segment of the mip region of the Legionella pneumophila genome. When using the Legionella pneumophila Detection Kit for conventional PCR, the amplified PCR product is 275 bp long and can be rendered visible directly in the agarose gel. The conventional PCR internal control amplicon is 191 bp in size. The real-time PCR variant is designed such that the specific target probe fluoresces at 520 nm. The real-time PCR internal control is detected by another probe emitting fluorescent light at 600 nm.

• primer sets and nucleotides at optimized concentrations

• positive and internal control for easy result verification

• instruction manual

• storage box

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Protocols for: AquaScreen® PCR Detection Kits - detection of Legionella pneumophila

AquaScreen®Lp Conventional PCR Detection Kit Detection of Legionella pneumophila. Protocol

(catalogue numbers 34-1025 / 34-1100 / 34-1250)

AquaScreen® ALp-QP Protocol

(catalogue numbers 34-2025 / 34-2100 / 34-2025 / 34-4025 / 34-4100 / 34-4250)

Please note: all protocols off site are the responsibility of the products supplier

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Felföldi T. et al., (2010). Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary. Clinical Microbiology and Infection, 16: 89–92 10.1111/j.1469-0691.2009.02795.x.

Kirschner A.K. (2016). Determination of viable legionellae in engineered water systems: Do we find what we are looking for?

Water Research, 93:276-88. doi: 10.1016/j.watres.2016.02.016.

Rozwadowska B. et al., (2013). Application of a real-time PCR method for Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens detection in water samples. Polish Journal of Microbiology, 62(4):439-43.

Vogel J. et al., (2013). Impacts of Migratory Sandhill Cranes (Grus canadensis) on Microbial Water Quality in the Central Platte River, Nebraska, USA. Water, Air and Soil Pollution, 224:1576. doi: 10.1007/s11270-013-1576-3.

Yaradou D.F. et al., (2007). Integrated real-time PCR for detection and monitoring of Legionella pneumophila in water systems. Applied and Environmental Microbiology, 73(5):1452-6. doi: 10.1128/AEM.02399-06.

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AquaScreen® real-time PCR Detection Kit (Type One)- for real-time PCR with LightCycler®1/2, Opitcon®, ABI Prism® and related devices including Legionella spp.-specific probes, internal amplification and postive control.

AquaScreen® real-time PCR Detection Kit (Type Two)- for real-time PCR with SmartCycler®2, Opitcon®, ABI Prism® and related devices, including Legionella spp. -specific probes, internal amplification and postive control.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

• Aqua Screen® Frequently Asked Questions
• Aqua Screen® LP Protocol
• Aqua Extract® Protocol