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蚂蚁淘在线 / 品牌中心 / Athens Research / Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-J774-1-05

Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-J774-1-05

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货号：EZT-J774-1-05
浏览量：80
品牌：Athens Research
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Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

J774A.1 Cell Avalanche™ Transfection Reagent (mouse reticulum cell sarcoma)

J774A.1 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on J774A.1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for J774A.1 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of J774A.1 Cell Avalanche™ Transfection Reagent for transfection on J774A.1 Cells

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
EZT-J774-1-05J774A.1 Cell Transfection Reagent (mouse reticulum cell sarcoma)0.5ml£399.00QuantityAdd to Order

J774A.1 Cell Avalanche™ Transfection Reagent (mouse reticulum cell sarcoma)

J774A.1 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on J774A.1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for J774A.1 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of J774A.1 Cell Avalanche™ Transfection Reagent for transfection on J774A.1 Cells

EZ Biosystems

Cell Line Information:

Designations: J774A.1 Depositors: P Ralph Growth Properties: adherent Organism: Mus musculus Morphology: macrophage Source: Strain: BALB/cN Tissue: ascites Disease: reticulum cell sarcoma Cell Type: monocyte/macrophage Cellular Products: interleukin 1 beta lysozyme Isolation: La Jolla California, United States Isolation date: 1968 Applications: Biological response; transfection host Age: adult Gender: female J774A.1 cells are active in antibody dependent phagocytosis. Their growth is inhibited by dextran sulfate, PPD and LPS. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta (Il1b) is synthesized continuously by this line.

The Transfection Reagent:

J774A.1 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on J774A.1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for J774A.1 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of J774A.1 Cell Avalanche™ Transfection Reagent for transfection on J774A.1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Unique formulation-maximize transfection performance in J774A.1 Cells.
  • Extremely gentle to cells
  • Deliver single or multiple plasmids
  • Animal-free
  • Simplest and straightforward protocols
  • Compatible with serum
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on J774A.1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for J774A.1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this J774A.1 Cell Avalanche Transfection Reagent.
For Other Cells:
J774A.1 Cell Avalanche™ Transfection Reagent (mouse reticulum cell sarcoma) can also be used on the following cells with high transfection efficiencies.

RAW 264.7 Cell 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Research Use Only. Not for use in diagnostic or therapeutic procedures.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶 免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.